Question

Question (10) Concerning the use of haemocytometer to count living and dead cells stained with Trypan blue dye, which of the following is/ are true;

1. It is possible to count the cells at 100x magnification
2. Trypan blue stains living cells
3. Trypan blue is a fluorochrome that can only be visualised using a UV light microscope
4. If left for longer than 20 minutes, the Trypan blue dye would stain all the cells, living and dead
5. Another dye, such as Nile red could be used instead of Trypan blue

Answer 1

B and D are true

A is false because It is possible to count the cells at 10x magnification

C is false as visualization is possible in electron microscopy too

E is false because nile red stains membranes especially lipids