Homework Answers
According with the disk method, the bigger the halo, the more effective is the antibiotic. As you can see, the Tetracycline treatment produced the biggest allow without any resistant colony. So, the Tetracycline should be the best option against Staphylococcus aureus. In fact, it has been reported that Tetracycline is much more effective against S.aureus than in E.coli
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The following is staphylococcus aureus disk diffusion (Kirby-Bauer) antibiotic testing Which antibiotic could be selected as...
Upon performing a Kirby-Bauer disc diffusion test using Staphylococcus aureus, you observe no zone of inhibition...
Upon performing a Kirby-Bauer disc diffusion test using Staphylococcus aureus, you observe no zone of inhibition with penicillin and a 15mm zone of inhibition with vancomycin. Based on these observation, which of the following conclusions is valid? Staphylococcus aureus is resistant to penicillin and sensitive to vancomycin. Staphylococcus aureus is resistant to both vancomycin and penicillin. Staphylococcus aureus is resistant to penicillin but may or may not be sensitive to vancomycin. Penicillin is bactericidal toward Staphylococcus aureus, and vancomycin is...
Lab Report 6 Antimicrobial Activity Lab report 6 Antimicrobial Activity Observations Part A. Kirby-Bauer Antibiotic Sensitivity...
Lab
Report 6 Antimicrobial Activity
Lab report 6 Antimicrobial Activity Observations Part A. Kirby-Bauer Antibiotic Sensitivity Test Chemotherapeutic Gram negative Gram Positive agent E. coli S. aureus B. cereus Zone Susceptibility Zone Susceptibility Zone Susceptibility Size Size Size Penicillin Streptomycin Tetracycline Chloramphenicol Gentamicin Vancomycin Chemotherapeutic agent Spectrum of Activity (Broad or limited) Type(s) of Microorganisms (Gram positive or negative) Penicillin Streptomycin Tetracycline Chloramphenicol Gentamicin Vancomycin Part B. Synergistic effect of drug combinations Culture and drug combination Appearance of Zone Synergistic...
Kirby-Bauer Test time-lapse with E. coli and S. aureus Elapsed Time: 24 hours coll Il 0...
Kirby-Bauer Test time-lapse with E. coli and S. aureus Elapsed Time: 24 hours coll Il 0 :18/1:11 Ampicillin Gentamicin Neomycin Vancomycin Tetracycline Bacitracin Trimethoprim/ Sulfamethoxazole Optochin Penicillin Erythromycin Streptomycin Amoxicillin/ Clavulanic Acid E. coli HD Q : II 0 - 0:33/1:11 3. Which of your organisms appears more susceptible to penicillin? 4. Which of your organisms appears difficult to treat with the selected antibiotics? 5. What is the difference between G+ and G-in reference to the selected antibiotics? 6. Which...
can you please help me with Qs 1-6 thanks ! LAB EXERCISE #6: Kirby-Bauer Disk Diffusion...
can
you please help me with Qs 1-6
thanks !
LAB EXERCISE #6: Kirby-Bauer Disk Diffusion Test for Antibiotie Sensitivity Determination The Kirby Bauer Test is an agar diffusion test that is used to determine the effectiveness of antibiotics killing various species of bacteria. Filter paper disks saturated with the antibiotic of interest are placed a Mueller-Hinton agar plate on which bacteria that has been isolated from a clinical sample has been sarcad. The antibiotic then diffuses from the disk...
A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The...
A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The following is a summary of the assay performed: The clinician spread the surface of several Mueller-Hinton agar plates (a media type containing beef extract, casein, and starch) with the bacterial culture using aseptic technique. Next, they applied sterile antibiotic disks, each containing a different antibiotic to the surface of the plates. After the agar plates were incubated overnight, the clinician measured the diameter of...
The physician orders a lab technician to perform a Kirby Bauer disk diffusion assay and finds...
The physician orders a lab technician to perform a Kirby Bauer disk diffusion assay and finds that the organism is resistant to the following antibiotics: 1) Penicillin, 2) Ampicillin, 3) Methicillin, and 4) Trimethoprim/Sulfamethoxazole. The physician asks the man about any drug allergies and other pre-disposing factors that could impact anti-microbial therapy. The man is allergic to all of the beta-lactam antibiotics, all cephalosporins, and does not tolerate sulfa drugs well. The man also has a form of hepatitis from...
2. The MIC, Kirby Bauer and E-TEST are all tests that are used to determine which...
2. The MIC, Kirby Bauer and E-TEST are all tests that are used to determine which antibiotics work against specific bacteria. a. Your patient comes in with a Staphylococcus aureus infection and you know that tetracycline kills S. aureus, but you want to prescribe the patient with the lowest concentration of tetracycline, which test would you conduct to find the lowest concentration that killed off S. aureus? b. Your patient comes in with a mystery bacterial infection. You want to...
1.) Which antibiotic target could have the most toxicity associated with it? A. 50 S ribosomal...
1.) Which antibiotic target could have the most toxicity associated with it? A. 50 S ribosomal subunit B. folic acid synthesis metabolic pathway C. cell wall D. cell membrane 2.) You are hoping to discover a new antimicrobial drug that targets fungi. Which structure might you hope to have your antimicrobial target to minimize toxicity? A. ribosomes B. chitin C. peptidoglycan D. phospholipids of plasma membrane 3.) An antiviral drug that blocks the activity of the enzyme integrase is used...
3. Which of these antibiotics would be classified as narrow-spectrum antibiotics? grampos sneg) 4. Which of...
3. Which of these antibiotics would be classified as narrow-spectrum antibiotics? grampos sneg) 4. Which of these antibiotics would be classified as broad-spectrum antibiotics? lics? (will kill both 5a. Ampicillin is a form of penicillin. If the zone of inhibition for a susceptible strain of E. coli is mm, and for a strain of S. aureus is 29 mm, which one is inhibited by the lower concentration or ampicillin? 5b. Would you need a higher dose of ampicillin to cure...
Plate 1. Plate 2. MRSA 980 Plate 3. S. aureus MRSA Inhibition (dear)zone Measure in mm...
Plate 1. Plate 2. MRSA 980 Plate 3. S. aureus MRSA Inhibition (dear)zone Measure in mm Figure 2 Katelyn's diagram of how to measure a zone of inhibition from her microbiology lab notebook. Exercise1 Measure the zones of inhibition for each antibiotic on the plates shown in Figure 1 and note the measurements in the spaces in Table 1 below. (Note: The Kirby Bauer method is standardized so that no zone of inhibition is scored as a 0. and all...
Lab
Report 6 Antimicrobial Activity
Lab report 6 Antimicrobial Activity Observations Part A. Kirby-Bauer Antibiotic Sensitivity Test Chemotherapeutic Gram negative Gram Positive agent E. coli S. aureus B. cereus Zone Susceptibility Zone Susceptibility Zone Susceptibility Size Size Size Penicillin Streptomycin Tetracycline Chloramphenicol Gentamicin Vancomycin Chemotherapeutic agent Spectrum of Activity (Broad or limited) Type(s) of Microorganisms (Gram positive or negative) Penicillin Streptomycin Tetracycline Chloramphenicol Gentamicin Vancomycin Part B. Synergistic effect of drug combinations Culture and drug combination Appearance of Zone Synergistic...
Kirby-Bauer Test time-lapse with E. coli and S. aureus Elapsed Time: 24 hours coll Il 0 :18/1:11 Ampicillin Gentamicin Neomycin Vancomycin Tetracycline Bacitracin Trimethoprim/ Sulfamethoxazole Optochin Penicillin Erythromycin Streptomycin Amoxicillin/ Clavulanic Acid E. coli HD Q : II 0 - 0:33/1:11 3. Which of your organisms appears more susceptible to penicillin? 4. Which of your organisms appears difficult to treat with the selected antibiotics? 5. What is the difference between G+ and G-in reference to the selected antibiotics? 6. Which...
can
you please help me with Qs 1-6
thanks !
LAB EXERCISE #6: Kirby-Bauer Disk Diffusion Test for Antibiotie Sensitivity Determination The Kirby Bauer Test is an agar diffusion test that is used to determine the effectiveness of antibiotics killing various species of bacteria. Filter paper disks saturated with the antibiotic of interest are placed a Mueller-Hinton agar plate on which bacteria that has been isolated from a clinical sample has been sarcad. The antibiotic then diffuses from the disk...
A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The following is a summary of the assay performed: The clinician spread the surface of several Mueller-Hinton agar plates (a media type containing beef extract, casein, and starch) with the bacterial culture using aseptic technique. Next, they applied sterile antibiotic disks, each containing a different antibiotic to the surface of the plates. After the agar plates were incubated overnight, the clinician measured the diameter of...
The physician orders a lab technician to perform a Kirby Bauer disk diffusion assay and finds that the organism is resistant to the following antibiotics: 1) Penicillin, 2) Ampicillin, 3) Methicillin, and 4) Trimethoprim/Sulfamethoxazole. The physician asks the man about any drug allergies and other pre-disposing factors that could impact anti-microbial therapy. The man is allergic to all of the beta-lactam antibiotics, all cephalosporins, and does not tolerate sulfa drugs well. The man also has a form of hepatitis from...
2. The MIC, Kirby Bauer and E-TEST are all tests that are used to determine which antibiotics work against specific bacteria. a. Your patient comes in with a Staphylococcus aureus infection and you know that tetracycline kills S. aureus, but you want to prescribe the patient with the lowest concentration of tetracycline, which test would you conduct to find the lowest concentration that killed off S. aureus? b. Your patient comes in with a mystery bacterial infection. You want to...
3. Which of these antibiotics would be classified as narrow-spectrum antibiotics? grampos sneg) 4. Which of these antibiotics would be classified as broad-spectrum antibiotics? lics? (will kill both 5a. Ampicillin is a form of penicillin. If the zone of inhibition for a susceptible strain of E. coli is mm, and for a strain of S. aureus is 29 mm, which one is inhibited by the lower concentration or ampicillin? 5b. Would you need a higher dose of ampicillin to cure...
Plate 1. Plate 2. MRSA 980 Plate 3. S. aureus MRSA Inhibition (dear)zone Measure in mm Figure 2 Katelyn's diagram of how to measure a zone of inhibition from her microbiology lab notebook. Exercise1 Measure the zones of inhibition for each antibiotic on the plates shown in Figure 1 and note the measurements in the spaces in Table 1 below. (Note: The Kirby Bauer method is standardized so that no zone of inhibition is scored as a 0. and all...
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