You need 180 ng of DNA to set up a PCR. Your DNA extraction has a...

Question

Question

You need 180 ng of DNA to set up a PCR. Your DNA extraction has a concentration of 3ng DNA/ul. How much of my extraction do I need to use in the PCR?

science biology

Answer 1

Answer #1

Ans- desired DNA concentration for PCR=180 ng

DNA extraction concentration= 3 ng/ul

desired volume of extracted DNA, to set up a PCR= 180 ng / 3 ng/ul = 60 ul

you need 60 ul of your extracted DNA to set up a PCR

Answer 2

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