Option F scores best for appropriate pairs of primary and secondary antibodies that would likely to be most effective for this purpose since their emission and excitation Spectra do not overlap so accurate results would be obtained.
(17) Suppose that you are planning an immunofluorescent histochemical staining experiment to determine if protein X...
You have conducted an experiment to try to demonstrate that growth factor receptor X protein (GFRX) and ligand Y (LY) are expressed in the same cells using immunohistochemistry (IHC). You are using the following antibodies: Primary antibodies: Goat anti-GFRX Goat anti-LY Secondary antibodies: Horse anti-goat-FITC (FITC is a green fluorochrome) Donkey anti-goat TRITC (TRITC is a red fluorochrome) As you view your experiment under the appropriate fluorescent filter sets, you show that both labels (red and green) are expressed in...
AnswerB
only which I marked
Sample 4: Primary antibody: W6/32 (MHC class 1) Secondary antibody: rabbit anti-mouse FITC Condition: 4°C Please note: this image shows six cells at a smal scale than sample 3 Sample 3: Primary antibody: W6/32 (MHC class 1) Secondary antibody: rabbit anti-mouse FITC Condition: 22°C Please note: this image shows one single cell that has been enlarged Table 1 Experimental conditions and antibodies. Experimental conditions Primary antibody staining Secondary antibody staining Secondary Secondary antibody antibody type...
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EXPERIMENTAL PROCEDURES Animals All animals including pregnant ICR mice, neonatal Wistar rats, and NOD-SCID mice (8 weeks old, male) were purchased from CLEA Japan (Tokyo). All exper- imental procedures and protocols were approved by the Animal Care and Use Committee of Keio University and conformed to the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Cells Mouse ESCs were obtained from the Laboratory of Pluripotent Cell Studies, RIKEN...
explain figure 2 please on the third page
The Jounal of Immunol nes of the National Academy of Sciences USA 89. 6550-6554. 01992, by permision of Proc. Natl. Acad. Sci. USA Vol. 89, pp. 6550-63554, July 1992 Immunolog A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells RANDOLPH J. NOELLE时, MEENAKSHI ROY.. DAVID M. SHEPHERD., IVAN STAMENKOVICt JEFFREY A. LEDBETTER, AND ALEJANDRO ARUFFo Departmend Microbiology. Dartmouth Medical School One...