Question

Use the following figure to answer the next two (2) questions. In the structures shown, the nitrogenous ring component is an adenine. NH2 YO OA NH2 YO 5. Which one of the following E. coli enzymes is likely to be associated with the reaction shown in the figure? A. Primase RNA polymerase DNA polymerase III (A) and (B) (A), (B) and (C) E.

Answer 1

Answer - D, (A) and (B)

Explanation

0 0 0 ATP OH o= O= NH2 Too 0 0 Nucleotide Pyrophosphate

ATP $\rightarrow$ Ribonucleotide + Pyrophosphate

During the synthesis of RNA nucleotide, ATP is 1 of the four nucleotides incorporated directly into RNA molecules by RNA polymerase enzymes. Primase synthesizes RNA primers complementary to the DNA strand, so that the primer contain ribonucleotides not deoxyribonucleotides. DNA polymerase III only adds deoxyribonucleotides to the primary.

Here, the nucleotide formed is the ribonucleotide because oxygen is present on the 5th position of ribose sugar. DNA polymerase 3 can add deoxyribonucleotides not ribonucleotides.