Question

Case 2 In your immunology laboratory course, your professor has always used her serum as a negative control for the antinuclear antibody test. She had only 0.4 mL remaining, so she aliquoted 0.2 mL for the dilutions to be made for the students sitting at tables 1 and 2 in the lab class. For the students at table 3, her blood was drawn, and a new serum was prepared. Table 2 also used an aliquot of the new serum to have a second negative control because the students had an extra well-remaining. Although your professor’s samples from last year were negative, this year students at both table 2 and 3 see speckled staining at a 1:64 dilution of the professor’s serum sample. 1. What should be done next?

Answer 1

1. Observation of speckles is a positive reaction for the antinuclear antibody. If it is positive she was suffering from an autoimmune disorder. Furthur some more tests has to be conducted to find out to determine the disorder.