Question

i need help with this lab report effects of temperture/ enzyme concetration/ and pH on enzyme activity

those are the experemint paper and results

Exercise 5: Factors Effecting Enzyme Activity A. Testing the Effect of Temperature on Enzyme Activity The purpose of this exercise is to determine the effect of temperature on enzyme activity Materials Large plastic bin Reaction chamber with rubber stopper and tube to bom 50 mL graduated cylinder beliet o bs Filter paper mot o r.com Liver homogenate (hydrogen peroxidase source) and b e Forens odos los H:02 low nebo 10 boboto Procedure 1. Write a prediction based on how enzymes will respond to changes in temperature. 2. Divide tasks among the four group members. a. The bottle holder who initiates the reaction. b. The oxygen-level observer who reads the levels of oxygen produced. c. The timer who calls out the 5-second intervals for reading oxygen levels. d. The recorder who records the amount of oxygen produced. 3. Set up the experimental apparatus as shown in Figure 5.1.

Answer 1

1. Enzymes do not function optimally at low temperatures. The optimal temperature of enzyme actuacti lies between 20 to 37 degree celsius. At this temperature, the product released with increasing time, increases steadily.

2. For graphing, the x-axis is divided into 60 seconds, and the y axis is divided into 90 units (02 ml). Each point is plotted on the graph according to the data table, and the respective graphs are drawn.

TEST 40°C 23°e •17°C 20 20 40 50 50 60 30 40 Time (see)

3. Enzymes are biological catalysts that speed up the rate of a reaction. Enzymes belong to proteins (macromolecule).

pH prediction: Enzymes do not function well at very high or very low pH. The optimal pH for enzyme activity remains around pH 7. At low pH, little product is produced by the enzyme. At high pH, a moderate amount of product is produced by the enzyme.