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What color does at least 1 endospore positive microbe stain on an Acid Fast positive stain?...

What color does at least 1 endospore positive microbe stain on an Acid Fast positive stain? (a) purple (b) blue (c) "hot" pink (d) pink (e) green

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Solution-correct answer is option"e" green colour.

endospore shows green colour when stained with malachite green.

this stain is driven into cell to stain endospore becuase it has more affinity to endospore.

The extra stain is inside cell is washed out by water wash but stain of endospore is not washed out because endospore has more affinity to malechite green.

Then counterstain saffranin is used to stain cells vegetative part excluding endospore. so endospore shows green colour while other part of cell shows pink colour.

WHILE Application of Application of heat (mordant Application of Malachite Green (primary stain) water Application of Safrani

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