1. Western blotting: After western blotting process, the antibody detected too many non-specific bands, discuss possible reasons and how to improve the specificity.
2.If there was not any band, discuss possible reasons and how to troubleshoot?
3. If cultured cells were overgrown (1:2 seeding ratio), how to perform subculture experiment differently?
4. Determine the confluence of each of seeding ratio by estimation (1:10 and 1:20 ratio)
5. Procedure in cell subculture?
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1. Western blotting: After western blotting process, the antibody detected too many non-specific bands, discuss possible...
If cultured cells were overgrown (1:2 seeding ratio), how to perform subculture experiment differently? also, indicate Procedure in cell subculture.
Write down your analysis of this case on factors like 1. the negotiation process, strategy and tactics PACIFIC OIL COMPANY (A)* "Look, you asked for my advice, and I gave it to you," Frank Kelsey said. "If I were you, I wouldn't make any more concessions! I really don't think you ought to agree to their last demand! But you're the one who has to live with the contract, not me!" Static on the transatlantic telephone connection obscured Jean Fontaine's...