1) Draw a cartoon that shows the general mechanism of Glucocorticoid activation. Be sure to include other important proteins and cellular locations
2) Based on the cartoon, what do you expect using the GFP tagged Glucocorticoid Receptor to see when cells are treated with mifepristone?
1) Draw a cartoon that shows the general mechanism of Glucocorticoid activation. Be sure to include...
Not sure how to answer these immunology questions! Help! 1) An otherwise healthy person is involved in an accident that requires kidney transplantation within a week. As the attending immunologist you are aware of three family members (no identical twins) that are willing to donate a kidney. What would you do to prepare the patient to minimize rejection?1) Detail three tests you would use to determine your ultimate donor tissue. Outline the rational that you would use to choice your...
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DeepSea Explorer brings some sample near a hydrothermal vent on the seabed some 1000-meter deep. Upon examination with a light microscope, a unicellular star-shaped microorganism was discovered in the sample and cells were collected and their chemical compositions were analyzed. a) After analysis, scientists discover that these cells contain DNA, RNA, proteins and phospholipids Based on these results, you can conclude the organism is made of cells of Prokaryotic 1) Eukaryotic i) Cannot be determined b) Explain your reasoning...
As a continuation of the experiments in Question 1 you want to study the timing of the APC's activity more distinctly. To do this you treat your cells with MG132 - a proteasome inhibitor. When MG132 is added to cells proteins can still be ubiquitinated, but they are not degraded, because the proteasome is inhibited. In cells that are treated with MG132 you can monitor APC activity on Cyclin B using just the cyclin B a. antibody and a western...
1. Check all genes you would expect to be significantly expressed in eye cells. A.the RPSA gene B. the OPN1SW gene C. the OCT-4 gene D. the INS gene 2. Check all genes you would expect to be significantly expressed in stem cells. A.the RPSA gene B. the OPN1SW gene C. the OCT-4 gene D. the INS gene 3. Check all genes you would expect to be significantly expressed in pancreatic cells. A.the RPSA gene B. the OPN1SW gene C....
1) You are a studying the prog ression of cells through the cell cycle. You are particularly interested in the role of the APC, which in a ubiquitin E3 ligase, in the control of the metaphase cyclin (cyclin B) and the timing of events. To look at this you start by synchronizing your cells in G1 and examining the levels of Cyclin B over time. The APC usually works on cyclin B during anaphase. RBX57 is a substrate for Cylcia...
1. You set up a chemical reaction with two materials, but only one of which shows up by TLC (Compound A, Rf = 0.75). Your product is supposed to have an Rf of 0.5 in the same eluent. Draw three TLC plates that you expect to see at these times: 1) at beginning of reaction, 2) halfway through reaction, 3) when reaction is done. Try to keep the TLC plates no more than 5cm in height, and just label the...
ENDOCRINE CASE STUDY Make sure to use APA format, use in-text citations and appropriate references. Answer each question fully and completely: each question and answer should stand on its own. An otherwise healthy thirty-eight-year-old woman presents with eight months of feeling anxious and tremulous. She reports having experienced intolerance to heat, weight loss, weakness and palpitations. On exam her pulse is 118 and blood pressure is 160/88 mmHg. She had a mild lid lag, a moderately enlarged, non-tender thyroid, and...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing a KDEL sequence (GFP-KDEL). When GFP-KDEL is expressed in normal human fibroblasts and examined using fluorescence microscopy, the fluorescence appears diffuse across the cytoplasm. How would you explain this observations given that KDEL is supposed to be an ER-specific sorting sequence? A. This engineered GFP would not have a hydrophobic signal sequence to get it into the RER in the first place. B. The...
immunology
practing samples and not sure if my answers correct.
1. NK cells are effective against viral infections because many virus infected cells a. are susceptible to lysis b. are susceptible to phagocytosis c. show reduced levels of MHC class I molecules d. show reduced levels of MHC class II molecules e. none of the above 2. Arachidonic acid serves as a substrate in the lipoxygenase and cyclooxygenase pathways resulting in the production of a. histamine, leukotrienes b. leukotrienes, prostaglandins...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy (EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could...