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A standard plate count of a culture of E. Coli has 175 colonies on the plate...

A standard plate count of a culture of E. Coli has 175 colonies on the plate labeled 10^-6 what is the original cell density (OCD, in CFU/ml) of the original culture?
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Answer #1

The label 10-6 means that the original culture has been diluted 106 times.

Since volume is not specified in the question it can be assumed that the volume of culture used to spread on the plate is 1 ml ( so that we can get the answer in CFU/ml). (CFU- colony forming units)

There are 175 colonies in 1 ml of culture that is been diluted 106 times.
This means the original culture will have 106 times the number of microbes (CFUs) in the diluted culture

So the original cell density is 175 X 106 = 1.75 X 108 CFU/ml

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