Question

The pull down assay described in Tutorial 7 is similar to co-immunoprecipitation technique described earlier in this course. Which of the following are differences between the IP/co-IP techniques and the pull down assay? Select all that apply. The co-IP assay utilizes antibody specific to the target protein. The pull down assay is used to study protein-protein interactions. O A pull down assay enables researchers to test the binding affinity of protein fragments The detection method used is different, co-IPs use Western blot analysis and pull down assays use Coomassie blue stain.

Answer 1

The detection method used is different, co-IPs use Western blot analysis and pull down assays use Coomassie blue stain.