Question

WIL Jinju collects the following data for her experiment using Enzyme T. She added 1 mL of enzyme to 4 mL of substrate S and measured the absorbance at 410nm. Based on her data, you would make the following conclusion: Time (sec) First 30 60 90 120 150 180 200 M substrate 0.457 0.582 0.711 0.831 0.996 1.125 1.447 1.572 1.668 1.713 1.830 220 M substrate 0.501 0.613 0.705 0.827 1.012 1.206 1.398 1.532 1.674 1.709 1.899 210 240 270 300 a. A higher substrate concentration makes the initial velocity (v.) faster. b. A higher substrate concentration makes the initial velocity (v.) slower. C. A higher enzyme concentration makes the initial velocity (vo) faster. d. A higher enzyme concentration makes the initial velocity (v.) slower. e. It is not possible to draw any of the conclusions above from this data.

Answer 1

Answer- a. A higher substrate concentration makes the initial velocity (v0) faster

Explanation-

1. Jinju carried out enzyme assay of enzyme T where she added 1ml of enzyme to 4ml of substrate of two different concentrations (200$\mu$M and 220$\mu$M) in separate test sets and allowed to proceed reaction from 30sec to 300sec at the interval of 30sec.

2. After the reactions at specific time interval of incubation she got result that indicates that increasing substrate concentration makes initial velocity faster by observing optical density.

3. When 1ml of 200$\mu$M substrate is incubated with enzyme for 30sec the optical density is 0.582 and for 220$\mu$M substrate concentration the optical density is 0.613. This indicates that at high substrate concentration optical density increases that indicates product formation.

4. All time interval of 30sec shows that optical density for 200$\mu$M is lower than 220$\mu$M substrate concentration. All this results shows that at higher substrate concentration initial velocity of reaction increases that leads to increased product formation that is observed by taking OD at 410nm.