Question

B. The researchers decided to determine the CI of each of the mutants, again using the horse infection model. The results are summarized in the table be- low: Strains used No. of bacteria in inoculum (CFU mutant: CFU wt) No. of bacteria recovered from lymph nodes on day 3 (CFU mutant:CFU wt) 10:1012 102:10 NeMutl:wt NeMut2:wt 105:105 105:105 105:105 NeMut1:NeMut2 103:106

The researchers decided to determine the CI of each of the mutants, again using the horse infection model. The results are summarized in the table above.

Determine the CI for NeMut1:wt, NeMut2:wt, and NeMut1:NeMut2. Interpret your results. Compare the results obtained from the LD50 and ID50 values with those obtained from the CI studies.

Answer 1

- Accading to the following data, Loso is the lethal dose that is required to kill half of individuals. In this case':. LD50 for wh: bebwean có 2 and co- LO5o for mute. Ouer 10²5 2050 for muta: Over 10^9. This is the graphic in excel for wild type strain : x axis is the dose and y axis is the percent mortality. metali

percent mortality VS dose 120% 100% 80% 60% 40% 20% ALDSO 200000 400000 600000 800000 1000000