Homework Answers
1) E.coli found in LB/PGLO plates there is no plasmid
2) Transformed cells found in AMP/X-GAL/pGAL plates genetically transformed cell will be found plasmid takenup which is expressed of ampcilin
3) control plates using for interpret experimental results there one is with ampcilin ( sample+ media) another one control plates contain only (media)
4)we can compare both -DNA/+Amp this is control plates and another one is +DNA/+Amp is variable.so compared altered one high E.coli compared to un-altered one.
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1. On which plate(s) would you expect to find bacteria most like the E. coli on...
Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment...
Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment in the lab. During the experiment plates with bacteria were inoculated from +GLO and -GLO microfuge tubes (LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate). 14) Explain what kinds of bacterial growth you will find on each of 4 plates after incubation ((LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate): under normal light and...
Pre-la question 1. Is it possible to transform an organism? Which Organism is most appropriate 2....
Pre-la question 1. Is it possible to transform an organism? Which Organism is most appropriate 2. How can you confirm that organism has been genetically trudomed? 3. Predict the effect of ampicillin on the transformed and non-transformed E. coli cells? 4. What is the function of CaCl on Ecoli cells? 5. Define competent cells 6. What is the function of heat shock during transformation 7. On which of the plates would you expect to find bacteria most like the original...
You set up a transformation hoping that a strain of E. coli will take up a...
You set up a transformation hoping that a strain of E. coli will take up a plasmid containing an ampicillin resistance gene, making the strain able to grow on LB + amp. To see if the transformation worked, you set up four plates: (1) Untransformed E. coli cells on LB plate (2) Untransformed E. coli cells on LB + amp plate (3) Transformed E. coli cells on LB plate (4) Transformed E. coli cells on LB + amp plate. Your...
1. Fill in the table above with what you observe on your plates. 2. Bacterial transformation...
1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
1. In a transformation experiment, E. coli uptake pRGLO plasmids that carry GFP gene. The transformed...
1. In a transformation experiment, E. coli uptake pRGLO plasmids that carry GFP gene. The transformed bacterial cells are grown on an ampicillin-treated agar plate. Which of the following is considered as a vector? GFP gene C E. coli cell c ampicillin-treated agar plate 0.5 points QUESTION 2 1. What is the function of bla gene? Break down the ampicillin Regulate the transcription of GFP gene Express the fluorescent trait in bacteria Control the uptake of DNA molecules in bacteria...
QUESTION 7 Imagine you discovered a strain of E. coli lacking a gene necessary to synthesize...
QUESTION 7 Imagine you discovered a strain of E. coli lacking a gene necessary to synthesize the amino acid histidine (they are therefore his). You attempt to rescue the cells by transforming them DNA from a wild-type his "donor" strain, and you plate your transformed sample and all controls on plates lacking histidine (-His) as well as plates containing histidine (+His). Shown below are the results of your transformation. + indicates there was growth, and - indicates there was no...
.1)a) Why was it important that the –pGLO bacteria were found growing on the LB plate?...
.1)a) Why was it important that the –pGLO bacteria were found growing on the LB plate? Why would their absence create problems in interpreting our results? b) What two conclusions (one per plate) should you draw from the observation that –pGLO bacteria did not grow on the LB/amp plate, but that some +pGLO bacteria did? c). Why did we observe bacteria colonies that glowed under UV light when we inoculated +pGLO bacteria on an LB/amp/ara plate, but not when we...
gene transfer like conjugatione 5. What is calcium chloride transformation? What does it accomplish? Calcium Chloride...
gene transfer like conjugatione 5. What is calcium chloride transformation? What does it accomplish? Calcium Chloride trensformation increases the ability of a bacteria call to in corporale plasmid DNA which callows it to be genetically frensformed. If accomplishes binding op plasmid DNA IV. 6. Why would you expect to find cell growth on the Luria nutrient agar plate? (LB plate)? 7. Did you expect to find cell growth on the ampicillin Luria plate? Explain WHY or WHY NOT. (LB/amp plate...
would you expect transformed cells? Plate # Label Growth Transformed Cells Yes 2 yes +PGLO LBlamp...
would you expect transformed cells?
Plate # Label Growth Transformed Cells Yes 2 yes +PGLO LBlamp +PGLO L Blamplara |- PGLO LB/amp IPGLO LB NO NO
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
Pre-la question 1. Is it possible to transform an organism? Which Organism is most appropriate 2. How can you confirm that organism has been genetically trudomed? 3. Predict the effect of ampicillin on the transformed and non-transformed E. coli cells? 4. What is the function of CaCl on Ecoli cells? 5. Define competent cells 6. What is the function of heat shock during transformation 7. On which of the plates would you expect to find bacteria most like the original...
1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
1. In a transformation experiment, E. coli uptake pRGLO plasmids that carry GFP gene. The transformed bacterial cells are grown on an ampicillin-treated agar plate. Which of the following is considered as a vector? GFP gene C E. coli cell c ampicillin-treated agar plate 0.5 points QUESTION 2 1. What is the function of bla gene? Break down the ampicillin Regulate the transcription of GFP gene Express the fluorescent trait in bacteria Control the uptake of DNA molecules in bacteria...
QUESTION 7 Imagine you discovered a strain of E. coli lacking a gene necessary to synthesize the amino acid histidine (they are therefore his). You attempt to rescue the cells by transforming them DNA from a wild-type his "donor" strain, and you plate your transformed sample and all controls on plates lacking histidine (-His) as well as plates containing histidine (+His). Shown below are the results of your transformation. + indicates there was growth, and - indicates there was no...
gene transfer like conjugatione 5. What is calcium chloride transformation? What does it accomplish? Calcium Chloride trensformation increases the ability of a bacteria call to in corporale plasmid DNA which callows it to be genetically frensformed. If accomplishes binding op plasmid DNA IV. 6. Why would you expect to find cell growth on the Luria nutrient agar plate? (LB plate)? 7. Did you expect to find cell growth on the ampicillin Luria plate? Explain WHY or WHY NOT. (LB/amp plate...
would you expect transformed cells?
Plate # Label Growth Transformed Cells Yes 2 yes +PGLO LBlamp +PGLO L Blamplara |- PGLO LB/amp IPGLO LB NO NO
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