Question

What extra experiment that is NOT included would increase your confidence in the conclusions drawn from Figure 3? Answer:

Answer 1

The extra experiment needed was a quantitative measurement of tubulin via Fluorimeter.

The microscopic images show that the microtubule regrowth for 1.5 minutes, which can be corroborated by measuring the actual amount of fluorescent tubulin from the PolD1 (both normal and mutant versions) cells at 1.5 minutes using a Fluorimeter.