Question

1) In a batch culture, formic acid (HCOOH) is produced using bacteria. For each mole of glucose (C H206) utilized by the bacteria, 2.40 moles of cells (CH1.8000 1No.26), 1.70 moles of formic acid, 1.90 moles of gaseous CO2, and some H20 are formed. Oxygen (O2) and ammonia (NH3) were consumed. Glucose, ammonia and 18,350 kg of water were fed to the reactor and inoculated with the bacteria. Air is pumped into the reactor and during the entire process 6000 kg of off-gas was recovered. The final formic acid concentration should not exceed 3 wt%. a. How much glucose and ammonia are required? b. What is the percentage of gases in the off-gas? 2) For the above described set-up, when the bacteria is grown at 37°C, estimate the cooling requirements.

Answer 1

0614 0.02.14 ç Hizº + NH3 + Q _) 2.4 (chigos, Nozc.) +19.04.2004) +1,9 coz + X 110, Balance the reaction. Canbyn arlomacol. 6 21.7 + 1.9. EG G=6 CBalance) Apologen Cary 2y Co. 269- Ï 0, Gly so left side multiply o Nilly with only so anlegen equiel the tregon artins 12+ 0.624(3) = 214(1.8) +. 17 (2.) 42.43 13:872 = 4.32 4 3.4 +22. ao 3.076. brugen akomy - G+2 = 1224 + 3.4+ 3.9 of 3.076. 8 11.6 (not bolanie 2 So left side multiply a atom with 2.8 we balance the a final form of reaction Cafter balance ) LCC Hic@s +0,624 NH3 + 2.804 > 2.4 CcHug@ası No.z] +1.7 Ch.com) : + 1-4 Co, +3+o7c 4, 6. Given that 1.8, 350 mg of attens fed do the reactor Convest into index by divide with molecular weight moles of waler ded is a 18350 (IS) 18 Chaglicmos) = 1019., uy komo) SES

Glucosem, consument is Yi | 1 - 614 vs 1,9 brow to muy fement #16 2016 tola.331,01 % kmei) of a Glucose comored Proto tag , (by mealthplying frako esos woryght Cathorou mw= 180. Theol. Lothe GloucoseComisuseck to e. 231.41%ica = 59,655 kg of the lease] Hill, consumed 0.624 of MIHz --> Home of Glucose . L 331, 418 keno) Glucose x = 206.804 kenal Nota [nim, commed : 206..07 = 3515 kg of mots] OG. Bond X 19 3515