1. A DNA prep with an A260 of 0.1 has a concentration of - ug/ml. 2....

Question

Question

1. A DNA prep with an A260 of 0.1 has a concentration of - ug/ml. 2. A DNA prep with an A260 of 0.25 has a concentration of u

science biology

Answer 1

Answer #1

1. 5 micrograms|/ml.

2. 12.5 micrograms/ml.

Brief explanation of the answer is provided below, i.e. calculation part is shown below:

Given, en! OD 260 = 0.1 failution factor 21 (assume)] We know, ds DNA Concentration = 50 pa bomo yo Do so X diluhim a factor

Answer 2

Similar Homework Help Questions

Assume that 50 ug/ml solution of native DNA has an A260 of 1, calculate the concentration...

Assume that 50 ug/ml solution of native DNA has an A260 of 1, calculate the concentration of your DNA sample in mg/ml. Then, assuming that 10% of the onion cells mass are the nuclei and that 10% of the nuclei mass is DNA, calculate the percent yield of your isolated DNA. My DNA sample A260 = 0.2204
One of the common methods to quantify DNA is to measure A260 (UV absorbance at 260...

One of the common methods to quantify DNA is to measure A260 (UV absorbance at 260 nm) of DNA solution using a spectrophotometer. You took 10 µl of your plasmid DNA preparation and dilute with 990 µl of H2O. The A260of this diluted solution was 0.30. Calculate the concentration of your plasmid DNA preparation (before the dilution). It has been established that 50 µg/ml of double-stranded DNA gives A260 = 1.0. My plasmid DNA preparation is _____________________ mg/ml. From this...
Given values A260 value of the diluted sample = 0.126 Abs the concentration of DNA in...

Given values A260 value of the diluted sample = 0.126 Abs the concentration of DNA in my diluted sample = 6.3ug/ml Dilution factor = 20 MY QUESTION Calculate the concentration of DNA in your undiluted sample - (I got 126ng/ul as an answer but unsure if this is right) calculate the total mass of DNA you isolated *** show work for this pls

Damon has a stock chymotrypsin concentration of 1 mg/mL. The protocol states to add 0.1 ml...

Damon has a stock chymotrypsin concentration of 1 mg/mL. The protocol states to add 0.1 ml of appropriately diluted enzyme to the cuvette which already contains Tris buffer (1.5 mL) and BTEE (1.4 mL). What volume (in uL) of enzyme does he need for a final reaction concentration of 20 ug/mL?
7. You purify DNA from a miniprep and wis units/ml (1 unit = 1 hg of...

7. You purify DNA from a miniprep and wis units/ml (1 unit = 1 hg of DNA digested per hour u to ensure complete digestion), 5X reaction butter, (b-e) to calculate how to prepare a restriction enzym a. Using the A260, what is the DNA's concentration? na miniprep and wish to digest it with EcoRI. You have DNA with an A260 of 2.0, EcoRI at digested per hour under optimal conditions, but 10 units are usually used per reaction "...
For a 1cm pathlength, the absorbance at 260nm (A260) equals 1.0 for a 50 microgram/mL solution...

For a 1cm pathlength, the absorbance at 260nm (A260) equals 1.0 for a 50 microgram/mL solution of double stranded DNA. What is (i) the concentration and (ii) the amount of nucleic acid in 750 microlitre of a solution of double stranded DNA with an A260 of 0.46?

How do protein concentration ranges compare with the DNA value? (DNA] of 50 µg/ml give an...

How do protein concentration ranges compare with the DNA value? (DNA] of 50 µg/ml give an A260 of 1.0 Can you explain the difference?
you have a solution containing 9 ug/ul of DNA. How would you dilute this to 1...

you have a solution containing 9 ug/ul of DNA. How would you dilute this to 1 ug/ul? make your final volume 0.1 ml
"calculate the total mass of DNA that you isolated at the end of Part A" how...

"calculate the total mass of DNA that you isolated at the end of Part A" how would you go about doing this, the concentration in my diluted DNA sample was 6.3ug/ml, and my A60 value was 0.126 Abs i don't know if these numbers help.. part A I had to calculate the concentration of DNA in my undiluted sample to which I did Concentration (ug/mL) = (A260 – A280) dilution factor 50ug/mL C=0.126-0.060 20 50nguL= 66ng/uL and go that the concentration...

What can you conclude about a DNA sample that has an a260/a280 ratio of 2.10 and...

What can you conclude about a DNA sample that has an a260/a280 ratio of 2.10 and an a260/a230 ratio of 1.70