Question

Short answer. Write your answer in the space provided. (1 point each) 6. Order the steps for the preparation of growing bacterial cells on solid agar plate. . place the cells in the cold for short-term storage measure the ingredients and prepare the nutrient agar in a heat-resistant glassware pour the agar into a Petri dish and let it solidify autoclave at 121 °C for 15 psi the agar for 15 minutes transfer the bacterial cells to the agar in the Petri dish grow the cells at a stable temperature suitable for their survival Why is the 2nd step extremely important when inoculating a pure culture?

Answer 1

Ordes the steps for the Preparation of growing bacterial cells on solid agar plate, 1. Measure the ingredients and prepare the nutrient agar in a beat-resistant glass ware. 2. Auto clave at 121°C for 15 psi the agar for 15 mins. 3. Pour the agar into a petridish and let it Solidify. 4. Transfer the bacterial cells to the agerin 1. Petridish. S. Grow the cells at a stable temperature suitable . for the survival. 6. Place the cells in the cold for short-term storage. g. why and step extremly important when Inoculating a pure Culture? Answer! - 121°c is considered as a suitable temperature for an auto clave because at this temperature the bacterial spores do not survive while, simply bringing to a boiling temperature ise, 100°C doesn't ensure the sterilization, so some bacterial Spores may Survéve at 100°c while 1216 ensures no survival of bacterial spores. Therefore, to inoculate a pune culture autoclave at 121c for 15 psi the ager for 15 minutes is extremsly important.