Question

Biochemistry Question: What is the difference between using a monoclonal antibodies vs. using polyclonial antibodies when performing a Western Blot?

You are studying the regulation of Bioch4 by the kinase Genetikinase 1. Your colleague performs a western blot, using a monoclonal Ms anti Bioch4 antibody, to look at Bioch4 expression with increasing concentrations of the Genetikinase stimulator Punnett. He obtains the following results: (Punnett) 1 mM 2.5 mm 5 mm 10 mM OM Punnett 250 150 KD BioCh4 GAPDH 379 This leads him to believe that Genetikinase stimulation results in a decrease in Bioch4 expression. This is counter to your hypothesis that Genetikinase actually stimulates Bioch4 activity. Because of your excellent training, you recognize the issue and ask him to repeat the western blot using a polyclonal Rb anti Bioch4 antibody. Your colleague grumbles, but agrees so that he can prove you wrong. The results now look like the following:

Answer 1

2015 December 5 6 7 23 Sunday 14 15 16 21 22 23 17 24 93 In W.B why we use palyclonal antibody : Unstead of monoclonal antibody 20 Monoclonal antibody does not mean it is more specific. Monoclonal are made by identical immune cells that have monovalent affinety, one molecule of antibody can bind to each antigen molecule although the epitope is repetative. - Polyclonal binds on multiple epitopen of the same antigen. That gives higher signal, when we use palyclonal antibody. Sensitivity of antigen. detection is very high an palyelonal antibody. for palymorphic antigen, monoclonal antibody cannot recognize all of them because their epilope is not present uniformy. The tiction of polyclonal antibody (Titler means presence of antibodies and morasunement of how much antibody an organism has 24 Monday produced that recognize by a particular epitope) in a blood sample) in more highen than monoclonal antibody During the regulation of Biochy by kinase Genetikinase 1, Palyclonal Rb anti Biochy givers better result that monoclonal 98 anti Biochy for these reasons in western.blot analysis 2020-3-12 00:13