Question

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26. B. Which of the following enzymes is associated with the repression of transcription? methyl transferase DNA polymerasex acetyl transferase histone kinase none of the above Section 2 - True/False (2 points each) 27. TFIIB regulates tissue or cell type-specific transcription 28._1 Eukaryotes have three different RNA polymerases that each can transcribe non-protein coding genes. 29. T The P site of the ribosome refers to peptidyl. be For T heoph UND 30._F_The input genetic material for RT-PCR is total RNA br purified mRNA. 31. The helix-loop-helix DNA binding domain binds to DNA only after breaking the hydrogen bonds between base pairs and opening up the double helix 32. T A transcription factor can function as both an activator and repressor depending on the identify of its binding partner(s). 33. T One way a transcription factor can function as a repressor is by blocking the activation surface of a transcriptional activator 34. The probability of initiating transcription of a given gene depends on the integration of multiple inputs provided by transcription activators, repressors, co- activators, and co-repressors 35 F Wobble refers to the imprecise binding of a transcription factor to its target DNA sequence 36. T The 5' cap of mature mRNAs has no role during translation initiation 37. T Enhancers are gene regulatory elements that function in the expression of specific genes and can be located hundreds or even thousands of base pairs from the start site of transcription 38. Protein folding does not begin until after the completion of translation 39. General transcription factors regulate tissue or cell type-specific transcription

Answer 1

26) methyl transferase because this add methyl groups on histone which then recruits various other proteins which then represses the transcription.

27)false.

28)true because Pol I II AND III transcribe rRNA , mRNA and some sno RNA , and trna and 5s RNA respectively among which only mena is protein coding.

29)true.

30)yes because the mRNA is converted into cDNA by reverse transcriptase which is then amplified and measured.

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