Question

IN YOUROWN WORDS, briefly explain how gel electrophoresis works.

Answer 1

Gel electrophoresis is consist of gel which is mainly consist of polyacrylamide or agarose which forms network with pores by crosslinking.

The proteins or DNA then migrates through the pores.

Which protein or DNA IS smaller migrate faster than the larger ones because larger one will be interrupted more whereas smaller will migrate easily through these pores as pores size is more than proteins size. .

FIRST , the protein becomes unfolded by SDS Page which reduces the disulfide bond and disrupt the protein structure. For , DNA, heat is given to make it in simpler structure.

Then , SDS being a negatively charged molecules makes the protein chain uniformly negatively charged, and DNA is negatively charged as phosphate groups.

Then voltage is driven and two electrodes are used one is positive and one negative.

Protein and DNA migrate towards the positive electrode as it is now negatively charged .