Question

Compensations have to be performed when we use O All the options are correct 0 1 antibody tagged to a fluorophore O more than one antibody tagged to different fluorophores whose excitation spectra show spectral overlap O more than one antibody tagged to different fluorophores whose emission spectra show spectral overlap

Answer 1

Compensation is required for a flow cytometry experi- -ment because of the physics of fluorescence. A fluorochrome is excited, and emits a photon in a range of wavelengths. some of those photons spill into a second detector, causing single stained samples to appear double positive. We apply a random mathematical correction to our data to address this spillover - this is compensation. Two important characterestice determine the usefulness of fluorochromes for flow cytometry: their ability to be excited. by light of a certain wavelength (excitation spectrum) and their ability to emit light of a longer wavelength after excitation (emission spectrum). Compensation is necessary because the fluorescene emission of a fluorochrome is not monochromatic or even close. so, A compensation have to be performed when we use. - I antibody tagged to a fluorophore. * Bandpass filter - c. Transmits light in the range of specified wavelength short pass filter - D. Transmits light equal or shorter than the specified wavelength. Long pass filter - A Transmit light equal or longer than the specified wavelength.

lela olo oo If If cells cells are CD4+ and cost and co4 and CD8+ → T-helper cells. To cytotoxic cells. Dace to visibility coastant could
Due to visibility constraints I could not observe the cell markers clearly in the third plot so, I have given both the possibilities.