Question

Question 4 2 pts What is the main advantage of the CRISPRCas method over the RT-PCR method? CRISPR is more accurate than PCR CRISPR detects individuals that are sick and individuals that had the disease but are not sick anymore. The test can be performed in less than 1 hr where the sample was taken. CRISPR is less expensive than PCR Question 5 4 pts What are some differences between the PCR that occurs in the Sherlock protocol and the PCR that occurs in RT-PCR test? PCR in Sherlock protocol is isothermic (all the steps occur at the same temperature). PCR in Sherlock protocol can amplify more than one gene at the same time Dyes are added to RT-PCR PCR in RT-PCR is faster

Answer 1

4. Answer - CRISPR is more accurate than RT-PCR.

CRISPR technology is very simple, easy to use and cheap, besides being very accurate. In this technology, gene editing is a genetic engineering technique in molecular biology by which the genomes of living organisms may be modified. PCR is a technology for genotyping, cloning, mutation, sequencing, paternity testing, etc. It is a three-step reaction and takes a longer time.

5. Answer - PCR in SHERLOCK protocol is isothermic [ the kit allows clinicians to quickly and cheaply diagnose patient samples and track epidemics. Viruses in blood samples or saliva can be detected quickly by a simple kit, unlike a long processing step in simple PCR technology]

* PCR in SHERLOCK protocol can amplify more than one gene at the same time [ this enables one to distinguish related viral species from one another and identify clinically relevant mutations like that in the Zika virus, which has been associated with microcephaly.

* Dyes are added to RT-PCR.