Question

QUESTION 3 Which of the following light microscopes require staining to view details of a fixed specimen on a light background? Inverted Microscope All of these Phase Contrast Microscope Confocal Microscope Darkfield Microscope Brightfield Microscope QUESTION 4 Which of the following would be the proper use of a microscope? Select all that apply. Move the microscope by carrying it by the arm and the base. After use turn off the light and unplug the microscope After using the oil immersion lens clean the oil from objectives and stage with lens paper After use lower the stage completely and remove any slides QUESTION 5 The microscope you observed in the oil immersion video was able to stay in focus after switching from the scanning and low powe high/dry lens. The microscope's lenses are considered to be Al(The answer should be one word)

Answer 1

Question 3: Brightfield microscope

Explaination: Among all the optical microscope, brightfield microscope is the simplest and uses illumination technique. During this illumination technique, it transmit the white light and provide contrast in sample. Staining is used to increase contrast level.

Question 4: All are correct

Explaination: 1) While moving the microscope one should always handle it properly. It is advised to lift a microscope with both hands by grasp the arm of microscope with 1 hand and with another hand at base of it. This help to maintain proper balance without making any fault in microscope.

2) As microscopes are expensive as well as useful instrument, it should be handle with proper care and adviced guidelines. It is adviced to switch off the light and unplug with main power source for safety of the microscope.

3) Oil immersion lens is a technique used to increase resolving power in microscope. leaving oil immersion in the open air will cause debris and dust in the air to impair image quality of the lens. so it is cleaned by sand paper.

4) It is adviced not to let objective lens touch the slides. so after usages, click the lower lens into position and remove the slides.

Question 5: Brightfield microscope

Explaination: Parfocal

The objective lenes of microscope are parfocal which means the user does not need to refocus during switiching to higher power regardless when object is in the lower power.