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Date Biochemistry Survey Name Partner Answer the following questions: 1. Why did sucrose give a positive test with Benedicts
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Answer #1
  1. Sucrose is a non-reducing sugar and thus does not react with Benedict's reagent. But heating sucrose (usually with dil. HCl) breaks the glycosidic bonds in sucrose through hydrolysis and thus it gives a positive result with Benedict's solution.
  2. Starch gets hydrolysed also when heated with H2SO4 for a couple of hours hence it will also give a positive result with Benedict's solution. However it will no longer give a positive result with I2 assuming that the entire starch content of the solution gets hydrolysed.
  3. The answer will depend based on the sample you used for the experiment.
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