Homework Answers
Answer:
I would tell her that she missed the last two steps: Rapid decolorization with ethanol or acetone and counterstaining with Safranin respectively.
Reason: Cells containing LPS means gram negative bacteria have very thin layer of peptidoglycan and present beneath the periplasm unlike ta gram positive bacteria. Thus they cannot retain the crystal violet colour due to poor peptidoglycan composition if decolorization is done with ethanol or acetone. Then we have to counterstain it with Safranin which will give a reddish or pinkish colour to the cells.
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1. A fellow student showed you a gram stained slide where cells containing LPS were stained...
Steps of Gram Staining Bacteria containing thick cell wall Violet color Bacteria containing thin cell wall...
Steps of Gram Staining Bacteria containing thick cell wall Violet color Bacteria containing thin cell wall (LPS) Violet color Crystal violet treatment lodine Purple color Purple color Decolorization Purple color colorless Safranin Purple color pink 3. A fellow student showed you a gram stained slide where cells containing thick cell walls were stained pink. What would you tell her about the staining procedure? Why? 4. A fellow student showed you a gram stained slide where cells containing LPS were stained...
1. A fellow student showed you a Gram stained slide where cells containing thick cell walls...
1. A fellow student showed you a Gram stained slide where cells containing thick cell walls were stained pink. What would you tell her about the staining procedure? Why? 3. In transduction, bacterial DNA is transferred to a new cell when It binds to a receptor on the bacterial cell wall. an inducer triggers the host to produce new DNA the virus is brought in by endocytosis. it is injected by the virus 4. When the bacteriophage transfers bacterial DNA...
SWAU u sulide of the slide Questions 1. What color are Gram-positive cells supposed to be...
SWAU u sulide of the slide Questions 1. What color are Gram-positive cells supposed to be after a Gram-stain? What about Gram-negative cells? (2 pts.) a rum + - purple Gram -- pink 2. What characteristics can be determined in a Gram-stain? (2 pts) 3. List all of the things that can go happen during the Gram-staining process that could lead to an incorrect or poor result. (2pts.) 4. Why are direct gram stains ordered on clinical specimens? (2pts.) 5....
NEED HELP WITH ALL PLEASE. MICROBIOLOGY You have stained a bacteria using the Gram staining technique...
NEED HELP WITH ALL PLEASE. MICROBIOLOGY
You have stained a bacteria using the Gram staining technique what color would any endospores be if present? A. Colorless B. Green C. Pink D. Blue E. Purple 2. If you omitted the Safranin step in a gram staining procedure, what would be the color of a Gram-negative bacteria? A. Colorless B. Pink C. Green D. Purple E. Blue 3. Bacillus licheniformis would be what color and shape after a Gram staining? A. Purple...
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Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order, function of each chemical, the effect of cach chemical and how gram + and gram - bacteria would appear at each step Color reactions at each step of procedure. Know why, based on the differences in cell wall structure, that the gram + vs gram - bacteria stain either purple or pink. What washes off in the decolorizing step of a gram - bacteria?...
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5. You use the Gram stain to try to differentiate bacteria in a sample. You find...
5. You use the Gram stain to try to differentiate bacteria in a sample. You find that all of the cells are Gram negative, but the teaching assistant insists there are both Gram positive and Gram negative cells present in the sample you are given. You definitely smeared a loop of the sample onto the slide and your fingers are stained a little purple and also a little red from handling the sides of the slide. Give a reason why...
0/1 pts Incorrect Question 8 You have a mixture of gram positive and gram negative cells...
0/1 pts Incorrect Question 8 You have a mixture of gram positive and gram negative cells in culture. You prepare a slide from the culture and gram stain it, but when observing the sample using oil immersion, all cells appear purple. What is the likely technical reason why only purple- colored cells are observ ed? you forgot the mordant step O you killed the cells during heat fixation you forgot the decolorization step you forgot to add immersion oi
1.Describe what you observed in your plaque smear wet-mount, direct stained slide, and indirectly stained slide....
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1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin,...
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin, and any other terms you think are important to the technique. You do not need to re- write the lab procedure here please describe what is occurring conceptually to both gram + and gram-cells during the procedure. What would a gram positive cell look like under oil immersion if a researcher forgot to use crystal violet? Please provide a rationale for your response. 3....
Steps of Gram Staining Bacteria containing thick cell wall Violet color Bacteria containing thin cell wall (LPS) Violet color Crystal violet treatment lodine Purple color Purple color Decolorization Purple color colorless Safranin Purple color pink 3. A fellow student showed you a gram stained slide where cells containing thick cell walls were stained pink. What would you tell her about the staining procedure? Why? 4. A fellow student showed you a gram stained slide where cells containing LPS were stained...
1. A fellow student showed you a Gram stained slide where cells containing thick cell walls were stained pink. What would you tell her about the staining procedure? Why? 3. In transduction, bacterial DNA is transferred to a new cell when It binds to a receptor on the bacterial cell wall. an inducer triggers the host to produce new DNA the virus is brought in by endocytosis. it is injected by the virus 4. When the bacteriophage transfers bacterial DNA...
SWAU u sulide of the slide Questions 1. What color are Gram-positive cells supposed to be after a Gram-stain? What about Gram-negative cells? (2 pts.) a rum + - purple Gram -- pink 2. What characteristics can be determined in a Gram-stain? (2 pts) 3. List all of the things that can go happen during the Gram-staining process that could lead to an incorrect or poor result. (2pts.) 4. Why are direct gram stains ordered on clinical specimens? (2pts.) 5....
NEED HELP WITH ALL PLEASE. MICROBIOLOGY
You have stained a bacteria using the Gram staining technique what color would any endospores be if present? A. Colorless B. Green C. Pink D. Blue E. Purple 2. If you omitted the Safranin step in a gram staining procedure, what would be the color of a Gram-negative bacteria? A. Colorless B. Pink C. Green D. Purple E. Blue 3. Bacillus licheniformis would be what color and shape after a Gram staining? A. Purple...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order, function of each chemical, the effect of cach chemical and how gram + and gram - bacteria would appear at each step Color reactions at each step of procedure. Know why, based on the differences in cell wall structure, that the gram + vs gram - bacteria stain either purple or pink. What washes off in the decolorizing step of a gram - bacteria?...
5. You use the Gram stain to try to differentiate bacteria in a sample. You find that all of the cells are Gram negative, but the teaching assistant insists there are both Gram positive and Gram negative cells present in the sample you are given. You definitely smeared a loop of the sample onto the slide and your fingers are stained a little purple and also a little red from handling the sides of the slide. Give a reason why...
0/1 pts Incorrect Question 8 You have a mixture of gram positive and gram negative cells in culture. You prepare a slide from the culture and gram stain it, but when observing the sample using oil immersion, all cells appear purple. What is the likely technical reason why only purple- colored cells are observ ed? you forgot the mordant step O you killed the cells during heat fixation you forgot the decolorization step you forgot to add immersion oi
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin, and any other terms you think are important to the technique. You do not need to re- write the lab procedure here please describe what is occurring conceptually to both gram + and gram-cells during the procedure. What would a gram positive cell look like under oil immersion if a researcher forgot to use crystal violet? Please provide a rationale for your response. 3....
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