Question

Question 10 (1 point) (a) The complete Gal4 dimer Activation domain Gal4 DNA-binding domain lacz ON Gal4 site (b) Gal4 lacking the activation domain lacz OFF Gal4 site (c) LexA lacking the activation domain DNA-binding 77 domain lacz OFF LexA site (d) Gal4-LexA hybrid Gal4 activation domain LexA DNA-binding domain lacz ON LexA site (Multiselect) In any experiment, controls are essential in order to determine the specific effect of changing some parameter. In the figure above, which constructs are the "controls" for the experiment that establishes the principle that activation domains are modular and interchangeable? The complete Gal4 dimer LexA lacking the activation domain n Gal4 lacking the activation domain The Gal4-LexA hybrid

Answer 1

A control in an invivo or invitro experiment is the one, in which changes are mostly minimised. It contains everything in it. If others are having changes in them. They can be observed using this control.
Among the given proteins, the complete Gal4 dimer is the control, because remaining three of them have changes in them like lacking lex A activation domain, lacking activation domain and Gal4-lexA hybrid, which is not homodimer like complete Gal4 dimer.
Hence, first option is the correct answer.