Question

Transformation Predicted Results…indicate using blue or white AND either.. no colonies, a large amount of colonies, or 10-100 colonies (remember each colony began as a single cell).

Plate Examined	Expected Results (In Brief)
LB Plate Competent Cell Control
LB amp Transformation Control
LB amp “-“ Genomic Ligation
LB amp “+” Genomic Ligation

Answer 1

Answers

Plate Examined	Expected Results
LB Plate Competent Cell Control	Large number of colonies (no antibiotic so more growth even after dilutions). Only the host cells are plated.
LB amp Transformation Control	50-100 colonies based on dilution. This has cells transformed with uncut plasmids.
LB amp “-“ Genomic Ligation LB amp “+” Genomic Ligation	If the ligation did not happen, then the vector will be linear therefore the transformation rate swill be high based on those that managed to circularize and gives rise to blue colonies. Blue colonies will form because the Lacz gene will be intact. If the ligation happens, then the vector will be a recombinant and the transformation rate will be based on the insertion rates. And the recombinant colonies will appear white because of the interrupted lacZ gene.