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10 points. The thr and leu operons are 55% linked in the E. coli chromosome. A...
10 points. An E. coli strain with a Tn10 that is 75% linked to a leuA...
10 points. An E. coli strain with a Tn10 that is 75% linked to a leuA mutant allele (Leu-) is used to transduce wt E. coli to TetR on rich Tet plates. If 100 of the transductants are scored on minimal glucose Tet plates how many will be TetR Leu+ and how many will be TetR Leu-?
10 points. An E. coli strain with a Tn10 that is 45% linked to the wt...
10 points. An E. coli strain with a Tn10 that is 45% linked to the wt proВ locus (Pro+) is used to transduce an E. coli strain which contains a mutant proВ allele (Pro-) to TetR on rich Tet plates. If 100 transductants are scored on minimal glucose Tet plates how many will be TetR Pro+ and how many will be TetR Pro-?
A generalized transducing phage is used to transfer genes from a thr(+)bio(+)thi(+) donor strain of E....
A generalized transducing phage is used to transfer genes from a thr(+)bio(+)thi(+) donor strain of E. coli to a thr(-)bio(-)thi(-) recipient strain. When transductants selected on a medium containing thiamine and threonine are tested for growth on other media, many more colonies are found on media supplemented only with threonine than on media supplemented only with thiamine. No transductants survive on minimal media. What is the correct order for these three genes? *I have seen multiple different answers for this,...
6. Transposon mutagenesis was performed to identify genes that are involved in the biosynthesis of theA amino acid alanine. A wildtype culture of E. coli was infected with a defective phage carrying...
6. Transposon mutagenesis was performed to identify genes that are involved in the biosynthesis of theA amino acid alanine. A wildtype culture of E. coli was infected with a defective phage carrying Tn10 A and the culture was plated on LB-tet media to obtain tet-resistant colony forming units. (LB is a rich growth media for E. coli and contains all amino acids required for bacterial growth) Individual CFU were then transferred to a multiwell plate containing liquid LB-tet media and...
3. (7) Suppose that you have selected E. coli that can grow only when the medium...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
strain (thrleut his pro pan bio). At each gene, + . (5 points) One triply auxotrophic...
strain (thrleut his pro pan bio). At each gene, + . (5 points) One triply auxotrophic E.coli strain (thr leur his pro pant bio*) is transforme ONA from a second triply auxotro ndicates prototrophy. - auxotrophy. After transformation, bacteria are plated on complete the master plate) and then replica plated onto minimal medium or minimal media with add nutrients as indicted in the figure below (recall that replica plating means that the positio colonies is the same in all plates)....
10 points. An E. coli strain with a Tn10 that is 75% linked to a leuA mutant allele (Leu-) is used to transduce wt E. coli to TetR on rich Tet plates. If 100 of the transductants are scored on minimal glucose Tet plates how many will be TetR Leu+ and how many will be TetR Leu-?
10 points. An E. coli strain with a Tn10 that is 45% linked to the wt proВ locus (Pro+) is used to transduce an E. coli strain which contains a mutant proВ allele (Pro-) to TetR on rich Tet plates. If 100 transductants are scored on minimal glucose Tet plates how many will be TetR Pro+ and how many will be TetR Pro-?
6. Transposon mutagenesis was performed to identify genes that are involved in the biosynthesis of theA amino acid alanine. A wildtype culture of E. coli was infected with a defective phage carrying Tn10 A and the culture was plated on LB-tet media to obtain tet-resistant colony forming units. (LB is a rich growth media for E. coli and contains all amino acids required for bacterial growth) Individual CFU were then transferred to a multiwell plate containing liquid LB-tet media and...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
strain (thrleut his pro pan bio). At each gene, + . (5 points) One triply auxotrophic E.coli strain (thr leur his pro pant bio*) is transforme ONA from a second triply auxotro ndicates prototrophy. - auxotrophy. After transformation, bacteria are plated on complete the master plate) and then replica plated onto minimal medium or minimal media with add nutrients as indicted in the figure below (recall that replica plating means that the positio colonies is the same in all plates)....
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