Homework Answers
1. Staphylococcus aureus is a Gram-positive, round-shaped bacterium. The answer is C. Because it can ferment mannitol and If an organism can ferment mannitol, an acidic byproduct is formed that will cause the phenol red in the agar to turn yellow.
2. E. coli is a Gram-negative rod-shaped bacteria, which possesses adhesive fimbriae and a cell wall. The answer is B.
3. Staphylococcus aureus can ferment mannitol.
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microbe A 1. Which of these microbes would best represent Staphylococcus aureus? One of these bacteria...
EXPERIMENT 1: Which bacteria was gram-positive after Gram staining? Select one: O a. S. aureus O...
EXPERIMENT 1: Which bacteria was gram-positive after Gram staining? Select one: O a. S. aureus O b. S. pneumoniae o cnone of the answer choices o d. E. coli EXPERIMENT 1: Which bacteria was pink and rod-shaped after Gram staining? Select one: O a. S. pneumoniae o b. E. coli O c. M. phlei O d. S. aureus EXPERIMENT 2: Which bacteria did not stain green after acid-fast staining? Select one: O a. E. coli O b. M. phlei O...
All of the following are characteristic of Staphylococcus aureus EXCEPT 1- produces coagulase 2- ferments mannitol...
All of the following are characteristic of Staphylococcus aureus EXCEPT 1- produces coagulase 2- ferments mannitol 3- grows on an MSA plate (i.e., halotolerant) 4- grows in chains You have isolated gram-positive cocci from a throat culture that you cannot identify as staphylococci or streptococci. A test for one enzyme can be used to distinguish these bacteria quickly. What is the enzyme? 1- dogalase 2- catalase 3- lactamase A candle jar is used to culture Streptococci because they 1- grow...
Multiple Choice. Highlight the single correct answer choice. 1. Streak plates are useful in microbiology to...
Multiple Choice. Highlight the single correct answer choice. 1. Streak plates are useful in microbiology to __________. quantify the number of bacteria measure turbidity identify bacteria determine cell shape 2. In the streak-plate technique, the intent is to isolate bacteria by dilution in theory by __________. dilution on a solid surface separating cells within the solid surface using a pipette dilution in water blanks 3. A pure culture consists of which of the following? one genus of microbe one species...
What is one structural difference between Staphylococcus aureus (Gram-positive bacterium) and Escherichia coli (Gram-negative bacterium) that...
What is one structural difference between Staphylococcus aureus (Gram-positive bacterium) and Escherichia coli (Gram-negative bacterium) that would make them have different susceptibilities to antimicrobial agents?
One of the following bacteria is in a sample: Staphylococcus aureus, Citrobacter freundii, Bacillus subtilis, Proteus...
One of the following bacteria is in a sample: Staphylococcus aureus, Citrobacter freundii, Bacillus subtilis, Proteus vulgaris and Enterococcus faecium. After Gram staining and visualization of the sample by brightfield microscopy, the identification of the bacterium was narrowed to two possibilities. An indole test was performed on the bacterium and produced a negative indole test result. This was enough information to determine the identity of the microorganism. Which microorganism was in the sample? (2 pts)
Lab Report-Carbohydrates 1. Purpose 2. Special Media for Isolating Bacteria (Lab #12) a. Why are dyes...
Lab Report-Carbohydrates 1. Purpose 2. Special Media for Isolating Bacteria (Lab #12) a. Why are dyes such as phenol red, eosin or methylene blue added to the media? b. How does the bacterium change the media (i.e color of agar or colonies) after incubation? C. In this experiment, which media are selective, and which are differential? d. How did the results observe on the mannitol salt agar and EMB agar correlate to the Gram reaction of the bacteria? e. What...
1. Based on your results and the information in the lab manual, fill in the table...
1. Based on your results and the information in the lab manual, fill in the table below. Predicted Gram Reaction Ability to ferment lactose/sucrose Ability to ferment mannitol Coliform or Non-coliform S. aureus E. coli P. aeruginosa M. luteus 2. What general type of growth medium would you use to: (a) grow one type of bacteria but inhibit the growth of another type? (b) discriminate between different types of bacteria? 3. Why is it necessary to sterilize the loop between...
Q14. In the lab info it says that as a rule, most microbes can adjust to...
Q14. In the lab info it says that as a rule, most microbes can adjust to a range of salt concentrations. 7/29/2019 Which cell structure allows microbes to physically withstand hypotonic environments? Cell wall MSA has two properties. It is both selective and differential. When bacteria grow on this media, there is a visible difference between bacteria that ferment mannitol, and the bacteria that do not See Figure 2 (a)-effects of selective/differential media. Q15. Which of the following is correct...
the unkown is staphylococcus aureus 5. Draw a flow chart to show how you identify the...
the unkown is staphylococcus aureus
5. Draw a flow chart to show how you identify the unknown among the following possible microbes using minimal number of tests. (You should be able to identify your unknown isolate based on gram stain morphology, and one or two additional test results. Other test results or growth characteristics of the unknown isolate may be used as additional evidences to support your identification.) (Only show the tests which are needed for identify your unknown and...
identify three unknown microbes from a set of 12 possible organisms using morphology and metabolic characteristics....
identify three unknown microbes from a set of 12 possible organisms using morphology and metabolic characteristics. These are the names. Names of Possible Unknown Organisms Escherichia coli Enterobacter aerogenes Proteus hauseri (or P. vulgaris) Alcaligenes faecalis Klebsiella pneumonia Staphylococcus aureus Staphylococcus epidermidis Enterococcus (Streptococcus) faecalis Kocuria rhizophila (or Micrococcus luteus) Bacillus subtilis Corynebacerium xerosis Bacillus cereus In order to identify unknown organisms from the list of twelve bacteria, you will need to develop three dichotomous keys, one for each of...
EXPERIMENT 1: Which bacteria was gram-positive after Gram staining? Select one: O a. S. aureus O b. S. pneumoniae o cnone of the answer choices o d. E. coli EXPERIMENT 1: Which bacteria was pink and rod-shaped after Gram staining? Select one: O a. S. pneumoniae o b. E. coli O c. M. phlei O d. S. aureus EXPERIMENT 2: Which bacteria did not stain green after acid-fast staining? Select one: O a. E. coli O b. M. phlei O...
Lab Report-Carbohydrates 1. Purpose 2. Special Media for Isolating Bacteria (Lab #12) a. Why are dyes such as phenol red, eosin or methylene blue added to the media? b. How does the bacterium change the media (i.e color of agar or colonies) after incubation? C. In this experiment, which media are selective, and which are differential? d. How did the results observe on the mannitol salt agar and EMB agar correlate to the Gram reaction of the bacteria? e. What...
1. Based on your results and the information in the lab manual, fill in the table below. Predicted Gram Reaction Ability to ferment lactose/sucrose Ability to ferment mannitol Coliform or Non-coliform S. aureus E. coli P. aeruginosa M. luteus 2. What general type of growth medium would you use to: (a) grow one type of bacteria but inhibit the growth of another type? (b) discriminate between different types of bacteria? 3. Why is it necessary to sterilize the loop between...
Q14. In the lab info it says that as a rule, most microbes can adjust to a range of salt concentrations. 7/29/2019 Which cell structure allows microbes to physically withstand hypotonic environments? Cell wall MSA has two properties. It is both selective and differential. When bacteria grow on this media, there is a visible difference between bacteria that ferment mannitol, and the bacteria that do not See Figure 2 (a)-effects of selective/differential media. Q15. Which of the following is correct...
the unkown is staphylococcus aureus
5. Draw a flow chart to show how you identify the unknown among the following possible microbes using minimal number of tests. (You should be able to identify your unknown isolate based on gram stain morphology, and one or two additional test results. Other test results or growth characteristics of the unknown isolate may be used as additional evidences to support your identification.) (Only show the tests which are needed for identify your unknown and...
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