|
S.No. |
Glucose |
Lactose |
Expression of Z |
Explanation |
|
I+P+O+Z+Y+ |
1 |
100 |
Expressed |
As glucose is in low amount and lactose is in high amount, therefore lac Z is expressed. |
|
I+P+O+Z+Y+ I+P+O+Z+Y+ |
2 |
200 |
Expressed |
As glucose is in low amount and lactose is in high amount, therefore lac Z is expressed. |
|
I-P-OcZ+Y+ I+P+O+Z-Y+ |
0 |
0 |
Expressed |
As Oc is dominant, cis-acting and constitutive in nature and due to mutation in lac operator doesn’t allow the binding of normal repressor with operator, therefor continuous expression takes place. |
|
I+P-O+Z+Y+ I-P+O+Z+Y+ |
1 |
100 |
Expressed |
I+ and P+ are dominant thus the expression of the lac Z takes place. |
|
I+P+OcZ+Y+ I+P-O+Z+Y+ |
1 |
100 |
Expressed |
As Oc is dominant, cis-acting and constitutive in nature and due to mutation in lac operator doesn’t allow the binding of normal repressor with an operator, therefor continuous expression takes place. |
|
IsP+O+Z+Y+ I-P+OcZ-Y- |
0 |
Expressed |
Lac Is is dominant and thus inducer gets changed and cannot bind to operator but as there is no inducer thus no expression can be there but Oc is dominant, cis-acting and constitutive in nature and due to mutation in lac operator doesn’t allow the binding of normal repressor with operator, therefor continuous expression takes place (thus expression takes place even in absence of lactose). |
|
|
I+P+O+Z+Y- I+P+O+Z+Y- |
Not expressed |
No expression, as no inducer is present. |
||
|
I+P+O+Z+Y- I+P+OcZ+Y- |
Expressed |
As Oc is dominant, cis-acting and constitutive in nature and due to mutation in lac operator doesn’t allow the binding of normal repressor with operator, therefor continuous expression takes place. |
21 A A A Aa AOE E ADA Eta In class Prokaryotic Gene Reg: lac operon...
1. The map of the lac operon is P-O-Z-Y. The promoter (P) region is the start site of transcription through the binding of the RNA polymerase molecule before actual mRNA production. Mutationally altered promoters (P-) apparently cannot bind the RNA polymerase molecule. Certain predictions can be made about the effect of p-mutations, Use your knowledge of the lactose system to complete the following table. Insert a "4" where an enzyme is produced and a "_" where no enzyme is produced....
The lac operon in E. coli is a well-studied gene system, and
β-galactosidase (β-gal) is the product of the lacZ gene. The
diploid conditions represent the addition of a plasmid carrying
different components of the lac operon. Determine if β-gal will be
generated under the conditions. Assume that glucose is absent. A +
in the genotype indicates a functioning gene, while a – indicates a
loss-of-function allele. The OC is an operator mutant that cannot
bind the lacI protein. Use...
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene. The production of B-galactosidase (+ present or - absent) is measured when lactose is present and absent from the medium. Assume the mutations involve only 1, 0, or Z. A merodiploid is constructed for each of the three strains. The plasmid carries a wild type lac operon and lacl gene. The production of functional B-galactosidase (+ present or - absent) is measured when lactose...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the F' plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the F' segment...
A lac operon mutant is grown in the presence and absence of lactose and tested for activity of the biosynthesis genes ‘Z’, ‘Y’, and ‘A’. Given the genotype below, which biosynthetic genes would be expressed in the presence of lactose? I+ P+ O+ Z+ Y- A+/ F’ I- P+ O+ Z+ Y- A+ Select one: a. none b. Z, Y, and A c. P and O d. Z and A e. I, P, O, Z, Y, and A
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the P plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the P segment...
(3 pts) There is a new mutation in the Lac/gene, called IN, that introduces a missense mutation in the DNA binding domain of the protein. IN can not bind to the operator. Predict the outcome of the following lac operon genotype for the expression of functional lac enzymes in the different conditions indicated. (Note: + = wildtype, - = no functional protein made.) IN O+P+ Z-Y+ - lactose + lactose b-gal (Z) Permease (Y) b-gal (Z) Permease (Y) + +...
Determine the outcome for the lac operon genotype shown below: I-P+O+Z+Y-/I+P+OcZ-Y+ Assume lactose is present.
A number of mutations affect the expression of the lactose operon in E. coir. I^- a mutant lac repressor that cannot bind the lac operator I^s a mutant lac repressor that cannot bind lactose O^c a mutant lac operator that cannot bind repressor Lacl P^- a mutant lac promoter that cannot bind RNA polymerase Z^- a mutant lacZ lost beta-galactosidase activity Y^- a mutant permease that cannot transport lactose into the cell Consider the following strains each with the indicated...
Most of what we know about the lac operon in E. coli has come from the genetic analysis of various mutants. Below is a list of mutants for regions of the operon. A + superscript indicates no mutation and normal function of that region, - indicates a knock out and no function of that region, c indicates the mutation resulted in constitutive action of that region. The effect of the mutation is determined by expression of the lacZ gene as...