Homework Answers
From left, band lane 1 to right band lane7, Last band lane 7 DNA have smallest size while the band lane 2 & band lane 6 have larger DNA
Approx value
Band 1 - 1600 bp
Band 2 - 1500 bp
Band 3 - 1600 bp
Band 4- 800 bp
Band 5- 800 bp
Band 6- 1500 bp
Band 7- 500 bp
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smaller fragment closer to the end of the gel. 4) What are the sizes of the...
RESULTS: Analyzing your data Answer the following questions: 1) How many bands do you see in...
RESULTS: Analyzing your data Answer the following questions: 1) How many bands do you see in the "unculanes? If there are more than one bad explain the nature or origin of the other bands? If there are more than one band can you 2) Why did you have to electrophorese uncut DNA? 3) What size of fragments were you expecting when you digested the known samples of pBR322 with the enzymes you used? 4) What are the sizes of the...
A1 is my DNA, Enzyme 1 BsrB1 P1 is known pBR322 enzyme 1 A2 is my...
A1
is my DNA, Enzyme 1 BsrB1
P1 is known pBR322 enzyme 1
A2 is my dna enzyme 2 RSa1
p2 is known pBR322 enzyme 2
Au is my dna uncut
Pu is know pBR322 uncut
ladder Pu Au P, A, P2 Az HAAAM .. - וור RESULTS: Analyzing your data Tobbul Avdinol Answer the following questions: 1) How many bands do you see in the "uncut" lanes? If there are more than one band can you explain the nature...
While your gel is running, discuss your expected results for each lane on the gel •...
While your gel is running, discuss your expected results for each lane on the gel • Remove the gel from the apparatus and image on a UV transilluminator. (Remember to only illuminate the UV light when the shield is in place). 3) Using the DNA ladder, estimate the experimental size of all bands observed in the sample lanes. (3pt) Gel Lane Plasmid Number of bands observed Estimated size (bp) of each band 2 (tube 1) A 3 (tube 2) B...
can someone explain throughly on how to find a-c??? thanks!!! The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel be...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
How did you EXPECT your uncut lane to look in the gel image? What ACTUALLY happened?...
How did you EXPECT your uncut lane to look in the gel image?
What ACTUALLY happened? What is a plausible explanation if there
was any discrepancy?
How did the observed BclI and EcoRI digest results compare to
the expected results? If they differed, list a potential reason
why?
BclI was incubated at 50°C, while EcoRI was incubated at 37°C.
Why was this necessary? What might you predict to occur if you
accidentally switched the temperatures?
3) Using the DNA ladder,...
Hi I have a problem with number 5, it involves gel analysis results. There are 2 parts, a,b,c. For A Im sure you need to make a graph with distance in (cm) on the vertical axis and log10 bp on the hor...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
You have been given a 1.0 kbp human genome fragment that does not contain any repeated...
You have been given a 1.0 kbp human genome fragment that does not contain any repeated sequences. Your plan is to use it as a probe for a Southern Blot containing your friend's genomic DNA. You prepare your gel by digesting with the following restriction enzymes in the following lanes: Lane 1: size marker Lane 2: EcoRI Lane 3: HindIII Lane 4: BamHI Your friend happens to be homozygous for the region you are probing. Does that mean you will...
(16 pts.) The gel shown below shows patterns observed for a STR (short tandem repeat) locus among...
(16 pts.) The gel shown below shows patterns observed for a STR (short tandem repeat) locus among four sets of parents (1-8) and four children (A-D). One child comes from each set of parents. 3. Why are there two bands for each parent? a. b. Although all the people shown here have two bands, it is possible (and not unusual) fora person to have only one band. Explain how this could happen. Consider earlier discussions on extensions to Mendelian genetics....
Please help me answer these, thank you! What is an auxotrophic bacteria? A bacteria that cannot...
Please help me answer these, thank you!
What is an auxotrophic bacteria? A bacteria that cannot complete a metabolic pathway after a pair-wise feeding experiment O A bacteria that cannot complete a metabolic pathway due to a defective enzyme. A bacteria that can use its metabolic pathways to photosynthesize. A bacteria that can use its metabolic pathways to produce prodigiosin. What is agarose gel electrophoresis? Using electricity to run macromolecules through a gel-like medium to separate by size Using electricity...
3. Suppose that you are asked to conduct a study to determine whether smaller class sizes...
3. Suppose that you are asked to conduct a study to determine whether smaller class sizes lead to improved student performance of fourth graders. If you could conduct any experiment you want, what would you do? Be specific. More realistically, suppose you can collect observational data on several thousand fourth graders in a given state. You can obtain the size of their fourth-grade class and a standardized test score taken at the end of fourth grade. Why might you expect...
RESULTS: Analyzing your data Answer the following questions: 1) How many bands do you see in the "unculanes? If there are more than one bad explain the nature or origin of the other bands? If there are more than one band can you 2) Why did you have to electrophorese uncut DNA? 3) What size of fragments were you expecting when you digested the known samples of pBR322 with the enzymes you used? 4) What are the sizes of the...
A1
is my DNA, Enzyme 1 BsrB1
P1 is known pBR322 enzyme 1
A2 is my dna enzyme 2 RSa1
p2 is known pBR322 enzyme 2
Au is my dna uncut
Pu is know pBR322 uncut
ladder Pu Au P, A, P2 Az HAAAM .. - וור RESULTS: Analyzing your data Tobbul Avdinol Answer the following questions: 1) How many bands do you see in the "uncut" lanes? If there are more than one band can you explain the nature...
While your gel is running, discuss your expected results for each lane on the gel • Remove the gel from the apparatus and image on a UV transilluminator. (Remember to only illuminate the UV light when the shield is in place). 3) Using the DNA ladder, estimate the experimental size of all bands observed in the sample lanes. (3pt) Gel Lane Plasmid Number of bands observed Estimated size (bp) of each band 2 (tube 1) A 3 (tube 2) B...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
How did you EXPECT your uncut lane to look in the gel image?
What ACTUALLY happened? What is a plausible explanation if there
was any discrepancy?
How did the observed BclI and EcoRI digest results compare to
the expected results? If they differed, list a potential reason
why?
BclI was incubated at 50°C, while EcoRI was incubated at 37°C.
Why was this necessary? What might you predict to occur if you
accidentally switched the temperatures?
3) Using the DNA ladder,...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
(16 pts.) The gel shown below shows patterns observed for a STR (short tandem repeat) locus among four sets of parents (1-8) and four children (A-D). One child comes from each set of parents. 3. Why are there two bands for each parent? a. b. Although all the people shown here have two bands, it is possible (and not unusual) fora person to have only one band. Explain how this could happen. Consider earlier discussions on extensions to Mendelian genetics....
Please help me answer these, thank you!
What is an auxotrophic bacteria? A bacteria that cannot complete a metabolic pathway after a pair-wise feeding experiment O A bacteria that cannot complete a metabolic pathway due to a defective enzyme. A bacteria that can use its metabolic pathways to photosynthesize. A bacteria that can use its metabolic pathways to produce prodigiosin. What is agarose gel electrophoresis? Using electricity to run macromolecules through a gel-like medium to separate by size Using electricity...
3. Suppose that you are asked to conduct a study to determine whether smaller class sizes lead to improved student performance of fourth graders. If you could conduct any experiment you want, what would you do? Be specific. More realistically, suppose you can collect observational data on several thousand fourth graders in a given state. You can obtain the size of their fourth-grade class and a standardized test score taken at the end of fourth grade. Why might you expect...
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