Question

Explain how energy is invested and produced by substrate level phosphorylation in glycolysis including the specific steps.

Answer 1

Glycolysis is a process in which stepwise ,enzymatic break down glucose into pyruvate .

Glycolysis consist of two major steps

1)preparatory phase and cleavage

2)oxidative and payoff phase .

preparatory phase and cleavage

it is fist phage of glycolysis in which glucose molecule is activated by phosphorylation and then cleaved into two molecules of triode phosphates.it consist again following step

A)phosphorylation :in this step glucose undergo phosphorylation with help of ATP in presence of hexokinase .

B)Isomerisation :glucose converted into fructose 6 phosphate.

C) Phosphorylation 2 : fructose phosphorylated by ATP.

Itvalso include cleavage and Isomerisation with respiratory substrate is form 2 molecules of 3 PGAL.

oxidative and phosphorylation (Harvest energy)

In this phase oxidation by removal of hydrogen takes place and then there is ATP generation.

Below step include : oxidation and phosphorylation, ATP generation 1, Isomerisation , dehydration,ATP generation 2.