Question

Question 8 (1 point) Above is a sequencing gel using the Sanger technique. The gel was loaded at the top of the figure. What is the sequence of the longest band in this gel (at least what you can figure from the gel)? O ATT ACG CTA AGG CGA TTC GAT CCT O AGG ATC GAA TCG CCT TAG CGT AAT OTAA TGC GAT TCC GCT AAG CTA GGA OTCC TAG CTT AGC GGA ATC GCATTA

Answer 1

Here the correct option is the 4th one i.e. TCC TAG CTT AGC GGA ATC GCA TTA

Explanation -

Sanger sequencing is a method of DNA sequencing. Here, the main principle behind sequencing is that in the given DNA template solution dNTPs, DNA polymerase, DNA primer and ddNTPs(di-deoxynucleotide triphosphate) are added. Because of the dNTP, the chain growth of the primer takes lace while when the growing chain encounters the ddNTP the chain growth halts. Again, the chain growth of the next primer takes place and again it is halted by ddNTP at some other point. Thus, a larger number of fragments are obtained and then these fragments are run into the electrophoresis gel and then result are analysed.

The interpretation of the sequence of the DNA is based on the fact that the bottom of the gel is positively charged and the upside of the gel is negatively charged. Then, if we want to know the sequence of the desired DNA segment we have to note down the sequence from the positive;y charged bottom end (5' end) and stops at the negatively charged upper end (3' end).

T. C G arteos otaISOUS atrods atout

So, the correct sequence of the given DNA segment is :

5' - TCC TAG CTT AGC GGA ATC GCA TTA - 3'