Homework Answers
A. Insufficient DNA used in the reaction
This is here I guess most suitable option because every other definitely causes error in restriction experiment.
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Which of the following is NOT a possible source of error for a restriction digestion experiment...
Day 1, You have isolated DNA from 30 individuals and performed restriction enzyme digestion. You have...
Day 1, You have isolated DNA from 30 individuals and performed
restriction enzyme digestion. You have loaded the product and run
the gel electrophoresis following the instruction properly. You
expected to see DNA bands after the procedure is completed, like
figure A, but after the completion of the run your gel looks like
figure B. You did not see any DNA band. You were so upset, but your
lab partner fixed the problem and your gel looked like figure A....
1. A circular plasmid has two PmeI restriction sites. A PmeI restriction enzyme will cut this...
1. A circular plasmid has two PmeI restriction sites. A PmeI restriction enzyme will cut this plasmid into two fragments. A. True B. False 2.In general, restriction enzymes that recognize four nucleotides have higher probability to produce more DNA fragments than those enzymes that recognize six nucleotides. A. true B. false 3. Which of the following sequences are palindromes? A. 5' TGGCCA 3' B. 5' GAAAAG 3' C. 5' CGATGG 3' D. 5' GACGAC 3' 4. Below are the possible...
i nee help analysing this data Anssume that the fractions you collected from the partial purification...
i nee help analysing this data
Anssume that the fractions you collected from the partial
purification by ionic chromatography were incubate with lambda DNA
and EcoR1 reaction buffer( Restriction enzyme reactions) and you
obtained the following bands in your agarose gel.
2 3 4 5 6 Lane Fraction Salt Content B and Lamda EcoR1 Marker 2 None 3 Lamda + fraction 2 Lamda + fraction 3 No salt No salt 21 3 4 5 7 8 9 10 6 6...
What are the possible source of error in this experiment? Experiment-> Molecular Weight of Polyvinyl Alcohol...
What are the possible source of error in this experiment? Experiment-> Molecular Weight of Polyvinyl Alcohol by Viscosity Select all that applies. Temperature fluctuations of the water bath Incomplete cleavage of the polymer The use of a manually activated stopwatch Partially blocked or unclean viscometer Proper filling of volumetric flasks
All of the following EXCEPT..... must be controlled in next week's experiment: A. the amount of...
All of the following EXCEPT..... must be controlled in next week's experiment: A. the amount of enzyme added to each tube B. the amount of substrate added to each tube C. the environmental parameter being tested D. the type of buffer used Next weeks experiment is about Environmental Parameters of Enzyme Activity. We will be determining how changes in temperature, pH, or salt concentration influences a lactase catalyzed reaction, the importance of cofactors and the effects of competitive inhibitors on...
What would happen if BsteII-HF had an optimal temperature of 30°C but we used the same...
What would happen if BsteII-HF
had an optimal temperature of 30°C but we used the same double
digestion protocol as we used in lab 4? (2pts)
Note: consider all the cloning steps for this
question
Double digestion of the the CAN1.Z ORNA and the PMD2 plasmid Bola I-HF SCHI CANL.Z! CANL.Z -SHI ©- MD2 CANLY → PMD2 CANLLY Bok I-HF You will cut both the plasmid PMD2 and the CAN1.Z ORNA using 2 enzymes (Bstell-HF and Sphl). These are two...
Assessing Some Possible Determinate Errors 1. One source of determinate error in this experiment would be...
Assessing Some Possible Determinate Errors 1. One source of determinate error in this experiment would be in determining the temperature of the gas in the buret. Based on the temperature of the reaction mixture in the flask before and after reaction, is the reaction exothermic or endothermie? Describe how this temperature change would affect the volume of the gas collected in the buret, and the calculated value of the gas constant. (1 mark) Explain how the calculated value of the...
2. hypothesize the best conditions (pH and temperature) under which the enzyme chymotrypsin functions with an...
2. hypothesize the best conditions (pH and temperature) under
which the enzyme chymotrypsin functions with an appropriate
reference. also, hypothesize what will occur in the presence of an
inhibitor and the type of inhibition.
EXPERIMENT 5: ENZYME ACTIVITY WITH a-CHYMOTRYPSIN Prelab Assignment 1. Prepare a flow chart, covering one half of the experimental work (either part A and C if your lab bench is on the window side of the lab or part B and D if your locker is...
I need the answers for questions 2 and 3. My DNA ladder is in lane 2...
I
need the answers for questions 2 and 3. My DNA ladder is in lane 2
marked by the yellow arrow. Thanks!
Here is the only other info I have. Thanks!
Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
What control elements regulate expression of the mPGES-1 gene? The promoter of a gene includes the...
What control elements regulate expression of the mPGES-1 gene? The promoter of a gene includes the DNA immediately upstream of the transcription start site, but expression of the gene can also be affected by control elements. These can be thousands of base pairs upstream of the promoter, grouped in an enhancer. Because the distance and spacing of these control elements make them difficult to identify, scientists begin by deleting sections of DNA that contain possible control elements and measuring the...
Day 1, You have isolated DNA from 30 individuals and performed
restriction enzyme digestion. You have loaded the product and run
the gel electrophoresis following the instruction properly. You
expected to see DNA bands after the procedure is completed, like
figure A, but after the completion of the run your gel looks like
figure B. You did not see any DNA band. You were so upset, but your
lab partner fixed the problem and your gel looked like figure A....
i nee help analysing this data
Anssume that the fractions you collected from the partial
purification by ionic chromatography were incubate with lambda DNA
and EcoR1 reaction buffer( Restriction enzyme reactions) and you
obtained the following bands in your agarose gel.
2 3 4 5 6 Lane Fraction Salt Content B and Lamda EcoR1 Marker 2 None 3 Lamda + fraction 2 Lamda + fraction 3 No salt No salt 21 3 4 5 7 8 9 10 6 6...
What would happen if BsteII-HF
had an optimal temperature of 30°C but we used the same double
digestion protocol as we used in lab 4? (2pts)
Note: consider all the cloning steps for this
question
Double digestion of the the CAN1.Z ORNA and the PMD2 plasmid Bola I-HF SCHI CANL.Z! CANL.Z -SHI ©- MD2 CANLY → PMD2 CANLLY Bok I-HF You will cut both the plasmid PMD2 and the CAN1.Z ORNA using 2 enzymes (Bstell-HF and Sphl). These are two...
Assessing Some Possible Determinate Errors 1. One source of determinate error in this experiment would be in determining the temperature of the gas in the buret. Based on the temperature of the reaction mixture in the flask before and after reaction, is the reaction exothermic or endothermie? Describe how this temperature change would affect the volume of the gas collected in the buret, and the calculated value of the gas constant. (1 mark) Explain how the calculated value of the...
2. hypothesize the best conditions (pH and temperature) under
which the enzyme chymotrypsin functions with an appropriate
reference. also, hypothesize what will occur in the presence of an
inhibitor and the type of inhibition.
EXPERIMENT 5: ENZYME ACTIVITY WITH a-CHYMOTRYPSIN Prelab Assignment 1. Prepare a flow chart, covering one half of the experimental work (either part A and C if your lab bench is on the window side of the lab or part B and D if your locker is...
I
need the answers for questions 2 and 3. My DNA ladder is in lane 2
marked by the yellow arrow. Thanks!
Here is the only other info I have. Thanks!
Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
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