Fst is the statistical value that depends on the variations occurring in the population of the species and the variation occurring within the species. Thus the whole frequency is calculated. As it has been stated that the value for the Fst for the mouse species which has been separated by the distance of 100 Km. Thus according to the formula and the fact mentioned above the distance between the mouse species will not play any role in changing the value for Fst thus even if the mouse species have been separated by the distance of 200 Km then also the value will remain constant.
That is the value of Fst will not change even if the distance is increased between the species. Hence, the value for Fst will be 0.03 (It can not exceed 1, it always have a range from 0-1)
Formula to calculate Fst= (Variations between population - Variation within population) / variation between population
+NO papulghans of a small mouse species are seperated but 100 Lm and Est = 0.03...
Refer to the figure below. You are studying a remote island
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