Question

1. Which graph(s) can be used to estimate a Km for an enzymatic reaction? select all that apply

2. Which graph(s) can be used to calculate an association constant for a protein binding its ligand? select all that apply

3. Which graph(s) can be used to estimate protein stability and to understand folding thermodynamics? select all that apply

4. Which graph(s) can be used to estimate the equilibrium constant for the dissociation of a protein-ligand complex? select all that apply

5. Which graph(s) can be used to estimate the rate of an enzymatic reaction when the enzyme is saturated with substrate? select all that apply

tructure.com/courses 1 fraction bound fraction unfolded 0.6 0.7 01- о 0.1 0.2 0.3 0.4 0.5 0.6 0.7 [B] (M) о 0.1 0.2 0.3 0.4 0.5 [B] (M) И І І І І 1.01 fraction bound initial velocity т 0.6 0.7 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 3) (M) о 0.1 0.2 0.3 0.4 0.5 [B] (M) E 1 initial velocity fraction unfolded о 0.1 0.2 0.3 0.4 0.5 0.6 0.7 IBLIMI о 0.1 0.2 0.3 0.4 0.5 0.6 0.7 IRI (M)

Answer 1

1) Graphs D and E can be used to calculate the Km value because both these graphs provide with the information of the velocity and the substrate concentration and Km can be easily calculated using the equation:

V = (Vmax [S]) ÷ (KM + [S}) where, V= initial velocity,{S} substrate concentration

2)The association constant can be calculated by considering a graph which encodes details of the bound ligand.So graph A or C may be used.

3)The stability and folding thermodynamics may be understood by considering graphs B or F as stability depends on the effeciency in folding.

4) Equilibrium is also a parameter that determines stability so graph B or F can be used and dissociation of a protein ligand complex can be calculated by A or C

5) The rate can be calculated by a graph that has parameters of velocity of enzyme action therefore D or E may be taken