Question

how do you find Ve

DATA FROM ION EXCHANGE COLUMN TABLE 9.6 TUBE # Azoo TUBE # 16 A200 10.366 10.170 10.084 10.054 10.046 10044 A .oo 1.316 0.606 0.290 0.170 0.148 0.142 om Алоо 10.025 0.006 0.0120002 10.142 -0.001 0.275 -0.003 0.125 -0.003 10.032 -0.002 0.008 0.003 0004 -0.003 0-003 -0.003 10.005 0.001 Lo-005 0.001 10.005 0.001 10.006 0.001 0-120 0.411 0.6692-358 | 29 15 30 MARY OF SAMPLE DATA ATEINS - PARTI TABLE 9.7 pH = 6, pH=6 AK FRACTION # SALT CONCENTRATION REQUIRED TO ELUTE TION BASED ON pl: IDENTIFY THE PROTEIN IN THE PEAK FRACTION #1 m) 15 Yes Ovalbumin ? #2 Qualbumin ?

Answer 1

Tube no.	Ve (eluted A400/A280)	A280	A400
1	0.24	0.025	0.006
2	0.166666667	0.012	0.002
3	-0.007042254	0.142	-0.001
4	-0.010909091	0.275	-0.003
5	-0.024	0.125	-0.003
6	-0.0625	0.032	-0.002
7	-0.375	0.008	-0.003
8	-0.75	0.004	-0.003
9	-1	0.003	-0.003
10	0.2	0.005	0.001
11	0.2	0.005	0.001
12	0.166666667	0.005	0.001
13	3.425	0.006	0.001
14	3.524663677	0.12	0.411
15	3.595628415	0.669	2.358
16	3.564705882	0.366	1.316
17	3.452380952	0.17	0.606
18	3.148148148	0.084	0.29
19	3.217391304	0.054	0.17
20	3.227272727	0.046	0.148
21	3.227272727	0.044	0.142

Best fractions are obtained from tube no. 13 to 21 as eluted material is higher as compared to rest of the tubes. [Note that Ve is the ratio of A400/A280]