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List the sequence of events that must occur to initiate transcription, beginning with an mRNA molecule...

List the sequence of events that must occur to initiate transcription, beginning with an mRNA molecule in the cytoplasm and ending with recruitment of the 2nd tRNA. Be specific about which ribosome sites are occupied.

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Answer #1

I think, by mistake you wrote transcription in place of translation in question ....

I will be explaining intiation of translation:

1)firstly, Initiation factor 3 restrict binding of 30s subunit to 50s subunit.

2)mRNA binds to 30s subunit with shine Dalgarno 16sRNA sequence which is complementary to leader sequence present in mRNA.

3)intiation factor 2 with GTP helps in attachment of N-formylmethionyl-tRNA to 30s subunit at p-site.

4) 70s ribosome form by joining of 30s and 50s subunit with hydrolysis of GTP.

5)then intiation factor 1 help in removal of GDP and intiation factor2.

6) out of three sites of ribosomes - donor site, acceptor site and exit site, 2nd tRNA with aminoacid(aminoacyl-tRNA) enters acceptor site with help of aminoacyl-tRNA-GTP-elongation factor-Tu complex.

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