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V R R V & EcoR1 8 , EcoR1, R & & PST1 PST1 Size Markers R & EcoR1 4 Calf Liver Calf & EcoR1 Liver 7 6 5 3 2 1 Wells 16mm Base

Now determine the sizes of fragments generated after digesting the r-plasmid with each of the restriction enzymes or combinations or restrictions enzymes.

R-plasmid digested with PstI:_______

R-plasmid digested with EcoRI:________

R-plasmid digested with PstI and EcoRI:__________

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Length of fragments are determoned in following image.

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