Question

infectious disease lab summary protocol. Questions that need to be answered are attached, aswell as the lab manuel

Name: ID: Protocol summary for: (Maximum 2 pages, Total = 10 marks) 1) Describe, in a sentence or two, the main objective(s) of this lab. (1) 2) What techniques will you be using in this lab? (2) 3) How many microorganisms will you be working with in this lab? (1) 4) Over the course of this lab (1", 24, ... week), which slides/plates/other material, already prepared for you (as mentioned in the lab sheets), will you be observing? (2) 5) Which slides/plates/other media will you be preparing/inoculating today (week 1)? (2) 6) Have you already used all of these techniques in this course? If not, please create a diagram, or list the steps, describing the procedure(s). (2)

Answer 1

prol to Main Objective of this lah are To study the urine samples in order to determine the infections of Urogenital System and microorepinisme reeponsible for it. 2. The techiques which we will be using are I a) Streaking I b) Plating, of mito. wine sample e) Inoculation d) Cream staining 3. Three bacterias are used to - study STD'S a) Neisseria sicca b) Neisseria flavescens () Escherichia coli

Date -/-/ 4. Let week. a) Obresving wine sample, in Kahn tube to inspect pH wing pH strips, turbidity, colour, odour. b) Plating the urine sample in Bloold Agar Plate & Mac Conkey colgar plate to select gram negative bacteria c) Gram Istaining of chocolate Agar or blood dear o grown cultures of Neisteria sicca & Neissellia flavescens, & Nutrient Agar glown Escherichia oli by loxidase reagent (dimethyl-p-phenylenediaminer and week a) Plate Colony counter, a Microscope for studying the physical appearence. I Hans-