Question

You are tasked with attempting to culture microbes in the lab that were initially obtained from samples taken in and near the Arctic circle, in areas of extreme cold and either very long or very short days, depending on the time of year. How would you begin to try to grow these microbes in the lab, and why would you choose those methods?

Answer 1

Growth of bacterial cultures is defined as an increase in the number of bacteria in a population rather than in the size of Endly idual cells. The growth of the bacterial population or cursu the geometric or exponential manner: with each division cylle I generation), one cell gives rises to 2 cells, the 4 cells, then & cells, then 16thon 32, and so forth. The time required for the formation of a generation, the generation time (a), can be calcuted forom the following formula it - t n 3.3 togb/Bt In the formula, B is the number of bacteria present at the start of the observation, b is the number of bresent after the time period it, and n is the number of gexstions generations. The relationship chows that the mean generation time is constant and that the rate at which the number of bacteria Increase is

proportimal to the number of bacteria at any given time. This relationship, Ps valid only during the period when the population le increasing in an exponential manner, called the log phase of growth. For this reason, graph that show the growth of bacterial cultures are plotted as the logarithm of the number of cells