13. Wollman and Francois Jacob formed a linkage map of E.coli
based on bacterial conjugation. They investigated the transmission
pattern of Hfr genes to F Negative cells. They concluded that gene
transfer occurs from a fixed point on the donor chromosome and
continues in a linear manner.
The explanation of this experiment is quite lengthy so if you want
to know about it please as in a different question separately.
14. False, it is not E.coli bacterium. It is in fact
bacteriophage. Bacteriophages are type of viruses that infect the
bacterial cells. You can see a more elaborate diagram on
google.
15
15. False, Mendel conducted his experiments before the discovery
of DNA and chromosome. Mendel has no idea that the heritable traits
that he was working on was intact genes and are in the form of
nucleotide chains. Mendel just looked at the phenotypic characters
and gave an idea about genotypes . Though there was no information
about genes back then, he was way ahead of his time in coming to
the conclusion that there are two alleles for every trait. Mendels
laws still hold true for the basics of genetics.
16. For transformation , the cells should be competent to take
up foreign DNA. After transformation the new DNA that is formed by
the process is called competent strand.
Heteroduplex is a DNA-RNA strand. Prophage is the DNA that has
phage genome incorporated in it.
17. Conjugation involves the F factor. As conjugation is the transfer of genetic material between to cells by a bridge like connection. This is called sexual reproduction or mating. F plasmid is important for pilus formation.
QUESTION 13 Elie Wollman and Francois Jacob (and others) developed a linkage map of E. coll...