Homework Answers
Answer: Option A is
correct
Explanation:
The EMSA method is used to determine the DNA binding properties of
proteins.
If a protein binds to a specific DNA sequence, it exhibits a
supershift in its position on the gel.
Competition with the unlabelled probe is performed to determine whether the DNA binding ability of the protein is specific or not.
If DNA binding is specific, the band intensity of the complex
(Protein with labeled probe) decreases with an increase in the
concentration of the unlabelled probe.
We can use a random/nonspecific oligo for the competition to confirm that it is indeed specific binding.
Question 15 1 pts Protein (nm) Complexها با با بن +Free DNA Lane 1 2 3...
Question 15 1 pts Protein (nm) Complexها با با بن +Free DNA Lane 1 2 3 4 5 6 7 8 In this figure what is it that is labeled? O False O Protein is labeled with an antibody O The DNA is end labeled O Both The DNA and the Protein are labeled It is impossible to tell from the figure.
Question 3 1 pts What protein must bind to the ORC complex in order to induce...
Question 3 1 pts What protein must bind to the ORC complex in order to induce DNA replication? Ubiquitin O p27 O Cyclin A Cdc25
please answer all 6 questions Question 27 3 pts TRBP is a protein important for the...
please answer all 6 questions
Question 27 3 pts TRBP is a protein important for the formation of the RISC complex. Which of the following would you expect in cells with null mutations in TRBP? o Reduced siRNA-mediated mRNA degradation o Increased miRNA-mediated translational repression o Increased deadenylase-mediated mRNA degradation o Reduced proteasome-mediated protein degradation D Question 28 3 pts A protein that binds to the 3' UTR of a VEGF mRNA and promotes deadenylation and uncapping is likely to:...
Question 1. 1 2 3 4 5 6 7 8 Lane Name/Code Ladder 1B | 2B...
Question 1. 1 2 3 4 5 6 7 8 Lane Name/Code Ladder 1B | 2B 2 DNA Sample/Treatment DNA ladder Digest Digest Digest Digest Digest Digest 3B 4B 5B Negative Figure 1: 1% Super buffer agarose gel electrophoresis of restriction digest of the plasmid containing the gdhA gene insert. Based on the information above answer the following questions? 1. What ladder size used? 2. What are the two top bands and bottom bands representing? 3. Explain why the observed...
Luestion 3 1 pts Review: You have the DNA that is radioactively labeled at the S'ends...
Luestion 3 1 pts Review: You have the DNA that is radioactively labeled at the S'ends of DNA as shown below. But you want DNA that is labeled only at one end of the DNA, not both ends. One of the other undergraduate students in the lab suggests that you use a restriction enzyme to cut the DNA, then electrophorese the DNA in an agarose gel, then cut out the region of the gel with radioactive DNA fragment you want,...
I just need the answers to questions 2 and 3. My DNA ladder is in lane...
I just need the answers to questions 2 and 3. My DNA ladder is
in lane 2 with the yellow arrow pointing to it. Thanks!
Part 2: Gel purification and ration Gel Slice and PCR Product Preparation modified from IBSci.com instructions for gel and PCR clean-up system A. Dissolving the Gel Slice 1. Following electrophoresis, excise DNA band from gel and place gel slice in a 1.5ml microcentrifuge tube. 1b. Use an analytical balance to weigh gel slice. Record weight...
I need help answering #5 and #6 on this worksheet ! im lost on what to...
I
need help answering #5 and #6 on this worksheet ! im lost on what
to do next ! thank you !
5. If someone working in your research lab approached you and said that they had been working on purifying a protein and were finally done. They had done gel filtration chromatography and made a graph to show their results - the Y axis measures absorbance... but it is for measuring light absorbed from several different wavelengths. The wavelengths...
I need the answers for questions 2 and 3. My DNA ladder is in lane 2...
I
need the answers for questions 2 and 3. My DNA ladder is in lane 2
marked by the yellow arrow. Thanks!
Here is the only other info I have. Thanks!
Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
1 pts DI Question 6 What region of this molecule shown would bind to mRNA during...
1 pts DI Question 6 What region of this molecule shown would bind to mRNA during translation? 3' A-OH 5' A Cacceptor stem G C G- U TuC loop D-loop C U GACAC m'A GGAGAGm m' G C-G A-U variable loop Anticodon loop Cm U A Gm A A The 5' end The anticodon loop The 3 end The variable loop The acceptor stem D Question 7 1 pts What is synthesized during transcription? O a strand of tRNA O...
I don’t understand, not sure if it’s true or false Question 16 1 pts If a...
I
don’t understand, not sure if it’s true or false
Question 16 1 pts If a "moderate" relationship exists between two variables in a population, we would be more likely to detect a statistically significant relationship between the variables with a sample size of 40 than with a sample size of 400. True O False
Question 15 1 pts Protein (nm) Complexها با با بن +Free DNA Lane 1 2 3 4 5 6 7 8 In this figure what is it that is labeled? O False O Protein is labeled with an antibody O The DNA is end labeled O Both The DNA and the Protein are labeled It is impossible to tell from the figure.
Question 3 1 pts What protein must bind to the ORC complex in order to induce DNA replication? Ubiquitin O p27 O Cyclin A Cdc25
please answer all 6 questions
Question 27 3 pts TRBP is a protein important for the formation of the RISC complex. Which of the following would you expect in cells with null mutations in TRBP? o Reduced siRNA-mediated mRNA degradation o Increased miRNA-mediated translational repression o Increased deadenylase-mediated mRNA degradation o Reduced proteasome-mediated protein degradation D Question 28 3 pts A protein that binds to the 3' UTR of a VEGF mRNA and promotes deadenylation and uncapping is likely to:...
Question 1. 1 2 3 4 5 6 7 8 Lane Name/Code Ladder 1B | 2B 2 DNA Sample/Treatment DNA ladder Digest Digest Digest Digest Digest Digest 3B 4B 5B Negative Figure 1: 1% Super buffer agarose gel electrophoresis of restriction digest of the plasmid containing the gdhA gene insert. Based on the information above answer the following questions? 1. What ladder size used? 2. What are the two top bands and bottom bands representing? 3. Explain why the observed...
Luestion 3 1 pts Review: You have the DNA that is radioactively labeled at the S'ends of DNA as shown below. But you want DNA that is labeled only at one end of the DNA, not both ends. One of the other undergraduate students in the lab suggests that you use a restriction enzyme to cut the DNA, then electrophorese the DNA in an agarose gel, then cut out the region of the gel with radioactive DNA fragment you want,...
I just need the answers to questions 2 and 3. My DNA ladder is
in lane 2 with the yellow arrow pointing to it. Thanks!
Part 2: Gel purification and ration Gel Slice and PCR Product Preparation modified from IBSci.com instructions for gel and PCR clean-up system A. Dissolving the Gel Slice 1. Following electrophoresis, excise DNA band from gel and place gel slice in a 1.5ml microcentrifuge tube. 1b. Use an analytical balance to weigh gel slice. Record weight...
I
need help answering #5 and #6 on this worksheet ! im lost on what
to do next ! thank you !
5. If someone working in your research lab approached you and said that they had been working on purifying a protein and were finally done. They had done gel filtration chromatography and made a graph to show their results - the Y axis measures absorbance... but it is for measuring light absorbed from several different wavelengths. The wavelengths...
I
need the answers for questions 2 and 3. My DNA ladder is in lane 2
marked by the yellow arrow. Thanks!
Here is the only other info I have. Thanks!
Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
1 pts DI Question 6 What region of this molecule shown would bind to mRNA during translation? 3' A-OH 5' A Cacceptor stem G C G- U TuC loop D-loop C U GACAC m'A GGAGAGm m' G C-G A-U variable loop Anticodon loop Cm U A Gm A A The 5' end The anticodon loop The 3 end The variable loop The acceptor stem D Question 7 1 pts What is synthesized during transcription? O a strand of tRNA O...
I
don’t understand, not sure if it’s true or false
Question 16 1 pts If a "moderate" relationship exists between two variables in a population, we would be more likely to detect a statistically significant relationship between the variables with a sample size of 40 than with a sample size of 400. True O False
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