When E. coli cells are grown in the presence of high glucose and high lactose, the lac repressor is not bound to the lac operator, cAMP levels in the cell are low and CAP is mostly not bound to the CAP binding site in the lac promoter. Under these conditions transcription of lac operon is at low level.
When E. coli cells are grown in the absence of glucose and presence of high lactose, the lac repressor is not bound to the lac operator, cAMP levels in the cell are high and CAP is mostly bound to the CAP binding site in the lac promoter. Under these conditions transcription of lac operon is at high level.
Fill in the blanks in the following sentences by selecting from the drop-down menus. When E.coli...
The lac operon contains a DNA sequence known as the lac promoter (P or P+ for wild type; P– for mutant (RNA polymerase does not bind)) that serves as the RNA polymerase binding site. The lac operon also contains a DNA sequence known as the Lac operator (O or O+ for wild type; O– or Oc for mutant (lac repressor cannot bind)) which is the binding site for lac repressor. The lac repressor, a protein, is encoded by the lac...
Please help me with this
question and if you can explain why.
Which of the following statements are true for bacteria grown in the absence of glucose and absence of lactose? Select all that apply. High levels of cAMP CAP bound to CAP binding site Low or no levels of allolactose O High levels of lacZYA mRNA No transcription of lacZYA Lac repressor bound to operator CAP is not bound to binding site O High levels of allolactose Lac repressor...
Fill in the blanks in this table with “yes” or “no” for each condition of lac operon regulation. The strain is wild type, with no partial diploidy. The first line is filled in for reference. Please explain your answers! Condition Lactose levels high? lac repressor bound to operator? cAMP levels high? CAP bound to CAP binding site? Transcription at highest level? high glucose no lactose no Yes no no no no glucose high lactose high glucose high lactose no glucose...
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c) Positive inducible d) Negative inducible 2) An activator regulatory protein… a) Is involved in negative regulatory control b) Is a cis acting regulatory element c) Recruits RNA polymerase to the promoter d) Prevents RNA polymerase from binding the promoter 3) For the lac operon, the presence of the substrate (lactose) activates the repressor. True or False? a) true b) false 4) cAMP… a) Activates...
The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
answer the following questions about lac operon: a) if the lack repressor protein is bound to the operator, are the E.coli metabolized lactose or not? explain your reasoning. b) If the lack repressor protein is bound to the operator and cellular glucose levels are low, is cAMP-CAP complex bound adjacent to the promotor, or not? explain your reasoning. c) if the lack repressor protein is bound to the operator and cAMP - CAP is bound to the CAP site, are...
all them please
Question 23 (1 point) The A and B alleles in ABO blood types can give rise to an individual that is blog type AB. This specific blood type is an example of: A) multiple alleles B) epistasis C) codominance D) partial dominance Imagine the gene encoding the lac repressor was mutated so that lactose (more technically allolactose) no longer bound to the repressor. However, the lac repressor was still capable of binding DNA at the operator sequence....
5. Jacob, Monod, and Pardee used various E.coli mutants to help determine how the lac operon is regulated. The descriptions of some of the mutants are listed. For each E. coli mutant determine if the lac operon would be on or off in a lactose-only environment. laci mutant: This mutant produces a super repressor that is not inactivated by allolactose. It will still stick. Operater will be off, lacO mutant: This mutant has an altered operator sequence so that the...
3. The CAP activator protein and the Lac repressor both control the Lac operon (see Figure 28-14). Fill out the table below with No expression, Low/Medium expression, or High expression to summarize when the Lac operon will be expressed in each of the three E. coli strains in the table. An example has been done for you. RNA polymerase- binding site (promoter) CAP binding site start site for RNA synthesis operator Lacz gene -80 -40 14080 nucleotide pairs Figure 08-14...
Imagine you are carrying out research on the lac
operon. You isolate six mutations in the lac operon by
measuring the amount of beta-galactosidase made in mutant cell line
under three different conditions: no lactose/no glucose; lactose
only; and lactose/glucose. Your results are shown in the table.
Strain
No Lactose, No Glucose
Lactose
Lactose, Glucose
Wild-type
None
High
Low
Mutant 1
None
None
None
Mutant 2
None
None
None
Mutant 3
None
Low
Low
Mutant 4
None
Low
Low
Mutant...