Question

1. There is a public debate as to why a passenger tests NEGATIVE with self-testing qualitative immunochromatography assay kit (cassette in) in a hotel room but tests POSITIVE with an Immunotechnique Assay called Immunofluorescence Assay (IFA) in an accredited laboratory upon arrival at the John F. Kennedy International Airport. You’ve been asked to educate the National Health Service and the general public

a. Mention 5 factors that could validate the POSITIVE test results from the laboratory

b. Explain the principle behind both test assays.

c. Mention 3 practical precautions for RDT self-testing

Answer 1

A) self assessment kits have less sensitivity than laboratory tests. Hence the chance for false negatives are high. Laboratory tests are carried out in proper environment without any contamination. Hence they are more accurate and sensitive.