Explain Focus-forming unit reduction assays (FFURA) as simple as possible (different from focus forming assay) a....
Explain Focus-forming unit reduction assays (FFURA) as simple as possible
(different from focus forming assay)
a. When is this method used?
b. Explain the protocol for it (in the simplest form as possible)
Homework Answers
Focus forming unit reduction assay(FFURA) is used to check the antiviral activity of substances like baicalin,a substance derived from Scutellaria baicalensis.FFURA is used to check the invitro antiviral activity.the percentage of foci reduction is obtained by comparing against untreated controls which are maintained in parallel
A)This method is used to evaluate in vitro anti dengueviral activity daily upto three days after the infection had occured
B)the protocol of FFURA:
Baicalein is added in the different phases of infection and the foci formed are compared with the untreated controls
the phases in which baicalein is added are: pretreatment,for 5 hours prior to infection for its prophylactic activity
during treatment,during virus adsorption,treatment for 4 days postadsorption
the percentage of foci reduction is then compared with the controls which are not treated with baicalein,and the antiviral activity is assessed
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Explain Focus-forming unit reduction assays
(FFURA) as simple as possible
(different from focus forming assay)
a....
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Please help and if possible explain a bit. Would help
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