Question

During hybridization, lowering the annealing temperature (select one)

Select one:

a. Increases specificity

b. Does not allow mismatches to form

c. Increases the stringency

d. Decreases the stringency

e. All of the above

Answer 1

Aringen ty a) Decreases the The pruimen ann caliy temperaturee primer at which hy breicli ze with the temp late DNA primer den aturation go cloon COLce we high T Too - speutr prcoduef cannealiny tempenatune, primerr cequenu the possibitily of non freom the upper imit, ow because at formaton n creeases wii specife and slighty unspetic he both tarcm pcoduct this preoclut stu'ctness f tracne steingeney Hene, fhe

It was a pleasure answering your question. Hope this helps...

Good luck!