Question

Clinicians and the public are excited about the prospects of replacing damaged and diseased tissues with patient-derived (autologous cells. Using autologous cells, as opposed to cells from a donor, avoids complications such as immune rejection. What series of steps could lead to the production of smooth muscle cells from the fibroblasts of a patient? Correct Answer List Question List (4 items) (Drag and drop into the appropriate area) First step Grow iPS cells in culture Use transcription factors to convert fibroblasts to iPS cells Use transcription factors to convertiPS cells to smooth muscle cells Obtain fibroblasts

place the steps in order

Answer 1

Obtain fibroblast cells

Use transcription factors to convert into iPS cells

Grow iPS cells im culture

Use transcription factors to convert iPS cells to smooth muscle cells

Process of autograft involves isolating the source cell that is fibroblast and converting into Induced pluripotent stem cells that can act as source for all cell types and growing IPS cells and than converting into the cell type to be transferred ( smooth muscle cells lls )